Now showing items 11-20 of 20
Direct chemical analysis of UV laser ablation products of organic polymers by using selective ion monitoring mode in gas chromatography/mass spectrometry
(Elsevier, 1994-02)
Trace quantitites of laser ablated organic polymers were analyzed by using commercial capillary column gas chromatography / mass spectrometry; the instrument was modified so that the laser ablation products could be ...
Development and characterization of a canine oral mucosa equivalent in a serum-free environment
(Wiley Subscription Services, Inc., A Wiley Company, 2004-10-01)
The objectives of this study were to develop a serum-free system for culturing canine oral keratinocytes, the construction and characterization of a canine ex vivo produced oral mucosa equivalent (EVPOME), and transduction ...
Classifications of ovarian cancer tissues by proteomic patterns
(WILEY-VCH Verlag, 2006-11)
Ovarian cancer is a morphologically and biologically heterogeneous disease. The identification of type-specific protein markers for ovarian cancer would provide the basis for more tailored treatments, as well as clues for ...
Toward high sequence coverage of proteins in human breast cancer cells using on-line monolith-based HPLC-ESI-TOF MS compared to CE MS
(WILEY-VCH Verlag, 2006-06)
A method is developed toward high sequence coverage of proteins isolated from human breast cancer MCF10 cell lines using a 2-D liquid separations. Monolithic-capillary columns prepared by copolymerizing styrene with ...
Hydroxyapatite implants with designed internal architecture
(Kluwer Academic Publishers; Springer Science+Business Media, 2001-06)
Porous hydroxyapatite (HA) has been used as a bone graft material in the clinics for decades. Traditionally, the pores in these HAs are either obtained from the coralline exoskeletal patterns or from the embedded organic ...
Validation of reverse phase protein array for practical screening of potential biomarkers in serum and plasma: Accurate detection of CA19-9 levels in pancreatic cancer
(WILEY-VCH Verlag, 2008-08)
The current study analyzed reverse phase protein arrays (RPPA) as a means to experimentally validate biomarkers in blood samples. One microliter samples of sera ( n 14= 1471), and plasma ( n 14= 1478) were serially diluted ...
A proteomic analysis of Psychrobacter articus 273-4 adaptation to low temperature and salinity using a 2-D liquid mapping approach
(WILEY-VCH Verlag, 2007-02)
Psychrobacter 273-4 was isolated from a 20 000–40 000–year-old Siberian permafrost core, which is characterized by low temperature, low water activity, and high salinity. To explore how 273-4 survives in the permafrost ...
Galanin inhibits rat pancreatic amylase release via cholinergic suppression
(Elsevier, 1992)
The effects of galanin on pancreatic exocrine function were examined using rat pancreatic tissues. In anesthetized rats, galanin (40 [mu]g/kg/h) decreased amylase secretion stimulated by 2-deoxy glucose (5.8 +/- 0.1 vs. ...
Automated integration of monolith-based protein separation with on-plate digestion for mass spectrometric analysis of esophageal adenocarcinoma human epithelial samples
(WILEY-VCH Verlag, 2006-09)
A unique approach of automating the integration of monolithic capillary HPLC-based protein separation and on-plate digestion for subsequent MALDI-MS analysis has been developed. All liquid-handling procedures were performed ...
Proteomic analysis of estrogen response of premalignant human breast cells using a 2-D liquid separation/mass mapping technique
(WILEY-VCH Verlag, 2006-07)
A 2-D liquid-phase separation method based on chromatofocusing and nonporous silica RP-HPLC followed by ESI-TOF-MS was used to analyze proteins in whole cell lysates from estrogen-treated and untreated premalignant, ...