Now showing items 51-60 of 83
Identification of proteins from two-dimensional gel electrophoresis of human erythroleukemia cells using capillary high performance liquid chromatography/electrospray-ion trap-reflectron time-of-flight mass spectrometry with two-dimensional topographic map analysis of in-gel tryptic digest products
(John Wiley & Sons, Ltd., 1999-10-15)
Protein spots from two-dimensional (2-D) gel electrophoresis of a human erythroleukemia cell line have been identified by analysis of the in-gel tryptic digests using capillary high performance liquid chromatography (HPLC) ...
Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry as a Rapid Screening Method to Detect Mutations Causing Tay–Sachs Disease
(John Wiley & Sons, Ltd., 1997-06-30)
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has been used as a rapid method for the detection of human genetic polymorphisms. In particular, the mutations in the human HEXA gene that cause the ...
Genetic Engineering and Therapy for Inherited and Acquired Cardiomyopathies
(Blackwell Publishing Inc, 2006-10)
The cardiac myofilaments consist of a highly ordered assembly of proteins that collectively generate force in a calcium-dependent manner. Defects in myofilament function and its regulation have been implicated in various ...
Direct transfer of a foreign MHC gene into human melanoma alters T cell receptor Vβ usage by tumor-infiltrating lymphocytes
(Springer-Verlag; Springer-Verlag Berlin Heidelberg, 1996-10)
The direct introduction of foreign genes into tumors shows promise as a therapeutic modality to enhance tumor immunogenicity. Hence, melanoma nodules were directly injected with a vector encoding an allogeneic MHC class ...
Red cells preserved with 10% hydroxyethyl starch: Effect of prefreeze washing
(Elsevier, 1975-10)
Prefreeze washing of red cells with saline and other wash solutions before freezing with 10% hydroxyethyl starch does not yield an improved product after thawing. The saline stabilities and cell recoveries are reduced and ...
Characterizing the cancer genome in lung adenocarcinoma
(Nature Publishing Group, 2007-12-06)
Somatic alterations in cellular DNA underlie almost all human cancers(1). The prospect of targeted therapies(2) and the development of high-resolution, genome-wide approaches(3-8) are now spurring systematic efforts to ...
Genotyping of Apolipoprotein E by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
(John Wiley & Sons, Ltd., 1998-08-31)
The genotyping of the various isoforms of Apolipoprotein E (apo E) has been performed using matrix-assisted laser desorption/ionization (MALDI-MS). The polymerase chain reaction was used to amplify the specific apo E gene ...
Validation of reverse phase protein array for practical screening of potential biomarkers in serum and plasma: Accurate detection of CA19-9 levels in pancreatic cancer
(WILEY-VCH Verlag, 2008-08)
The current study analyzed reverse phase protein arrays (RPPA) as a means to experimentally validate biomarkers in blood samples. One microliter samples of sera ( n 14= 1471), and plasma ( n 14= 1478) were serially diluted ...
Studies of Posttranslational Modifications in Spiny Dogfish Myelin Basic Protein
(Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media, 2001-05)
The objective of this investigation was to determine whether nonmammalian myelin basic protein contained charge isomers resulting from extensive posttranslational modifications as seen in mammalian MBP. Four charge isomer ...
Female Gender Attenuates Cytokine and Chemokine Expression and Leukocyte Recruitment in Experimental Rodent Abdominal Aortic Aneurysms
(Blackwell Publishing Inc, 2006-11)
Female gender appears to be protective in the development of abdominal aortic aneurysms (AAAs). This study sought to identify gender differences in cytokine and chemokine expression in an experimental rodent AAA model. ...