Bead size effects on protein-mediated DNA looping in tethered-particle motion experiments
dc.contributor.author | Milstein, J. N. | en_US |
dc.contributor.author | Chen, Y. F. | en_US |
dc.contributor.author | Meiners, J.-C. | en_US |
dc.date.accessioned | 2011-01-04T16:24:06Z | |
dc.date.available | 2012-02-21T18:47:01Z | en_US |
dc.date.issued | 2011-02 | en_US |
dc.identifier.citation | Milstein, J. N.; Chen, Y. F.; Meiners, J.-C. (2011). "Bead size effects on protein-mediated DNA looping in tethered-particle motion experiments." Biopolymers 95(2): 144-150. <http://hdl.handle.net/2027.42/78494> | en_US |
dc.identifier.issn | 0006-3525 | en_US |
dc.identifier.issn | 1097-0282 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/78494 | |
dc.description.abstract | Tethered particle motion (TPM) has become an important tool for single-molecule studies of biomolecules; however, concerns remain that the method may alter the dynamics of the biophysical process under study. We investigate the effect of the attached microsphere on an illustrative biological example: the formation and breakdown of protein-mediated DNA loops in the lac repressor system. By comparing data from a conventional TPM experiment with 800 nm polystyrene beads and dark-field TPM using 50 nm Au nanoparticles, we found that the lifetimes of the looped and unlooped states are only weakly modified, less than two-fold, by the presence of the large bead. This is consistent with our expectation of weak excluded-volume effects and hydrodynamic surface interactions from the cover glass and microsphere. © 2010 Wiley Periodicals, Inc. Biopolymers 95: 144–150, 2011. | en_US |
dc.format.extent | 302065 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Polymer and Materials Science | en_US |
dc.title | Bead size effects on protein-mediated DNA looping in tethered-particle motion experiments | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Physics, University of Michigan, Ann Arbor 48103 | en_US |
dc.contributor.affiliationum | Department of Biomedical Engineering, University of Michigan, Ann Arbor 48103 | en_US |
dc.contributor.affiliationum | Department of Physics, University of Michigan, Ann Arbor 48103 ; Biophysics in the College of Literature, Science, and the Arts, University of Michigan, Ann Arbor 48103 ; Department of Physics, University of Michigan, Ann Arbor 48103 | en_US |
dc.identifier.pmid | 20882535 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/78494/1/21547_ftp.pdf | |
dc.identifier.doi | 10.1002/bip.21547 | en_US |
dc.identifier.source | Biopolymers | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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