Now showing items 1-9 of 9
Concurrent unilateral chromatid damage and DNA strand breakage in response to 6-thioguanine treatment
(Elsevier, 1986-10-15)
The delayed cytotoxicity of 6-thioguanine (TG) may relate to the arrest of cells in G2 upon completion of one cell cycle after drug exposure. In Chinese hamster ovary (CHO) cells, both the unilateral chromatid damage in ...
Simultaneous determination of thymine and 5-bromouracil in DNA hydrolysates using gas chromatography--mass spectrometry with selected-ion monitoring
(Elsevier, 1986)
The gas chromatographic--mass spectrometric method using selected-ion monitoring (GC--MS--SIM) described here quantitatively determines the amount of DNA thymine replacement by 5-bromouracil (BU) after exposure to ...
Analysis of bromodeoxyuridine incorporation into DNA: Comparison of gas chromatographic/mass spectrometric, CsCl gradient sedimentation, and specific radioactivity methods
(Elsevier, 1987-02-15)
A sensitive new method for the quantitation of 5-bromodeoxyuridine (BrdUrd) incorporated into DNA by GC/MS analysis of enzymatically released Thy and bromouracil (BrUra) is presented. The hydrolysis procedure was characterized ...
Dynamics of Bromodeoxyuridine Incorporation into DNA of Squamous Carcinoma Cells During Mid and Late Logarithmic Growth
(Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media, 1988-08)
Head and neck squamous carcinoma cell lines, UM-SCC 1, 5, 9, 11B, and 14B, were exposed in vitro to bromodeoxyuridine (BUdR) during logarithmic growth to determine the effects of drug concentration (0.01 to 10 µ M ) and ...
Prediction of thioguanine-induced cytotoxicity by dual-parameter flow cytometric analysis
(Springer-Verlag, 1989-10)
A method is presented for the quantitative analysis of delayed cytokinetic effects resulting from the treatment of L1210 cells with 6-thioguanine (TG). By using dual-parameter (DNA/protein) flow cytometry, we could observe ...
N-acetylprocainamide is a less potent inducer of t cell autoreactivity than procainamide
(John Wiley & Sons, Inc., 1988-08)
We have reported that an inhibitor of DNA methylation, 5-azacytidine, makes cloned, antigen-specific CD4 + T cells autoreactive, and that procainamide and hydralazine mimic this effect. Those results suggested that ...
Enhanced cytotoxicity with methotrexate in conjunction with hypoxanthine in L1210 cells in culture
(Springer-Verlag, 1988-07)
By inhibiting dihydrofolate reductase, methotrexate (MTX) depletes cellular stores of reduced folates, resulting in the inhibition of DNA and RNA synthesis. Inhibition of RNA synthesis arrests cells in the G 1 phase of the ...
Direct measurements of thermal vacancies in BCC 4 He
(Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media, 1987-06)
X-ray lattice parameters of bcc 4 He crystals in contact with superfluid 4 He were measured with 300 ppm accuracy in the temperature range 1.494–1.711 K, corresponding to solid molar volumes between 20.88 and 21.09 cm 3 . ...
Dissimilar actions of 6-mercaptopurine and 6-thioguanine in Chinese hamster ovary cells
(Elsevier, 1985-10-15)
The actions of 6-thioguanine (TG) and 6-mercaptopurine (MP) were compared in Chinese hamster ovary (CHO) cells. Several differences were noted between these two agents. TG caused a greater maximal loss of clonogenicity, ...