Now showing items 1-10 of 10
Mechanism and pharmacological specificity of dUTPase-mediated protection from DNA damage and cytotoxicity in human tumor cells
(Springer-Verlag; Springer-Verlag Berlin Heidelberg, 1998-09)
Purpose : We have reported previously that the expression of E. coli dUTPase (dutE) can protect HT29 cells from 5-fluorodeoxyuridine (FdUrd)-induced DNA fragmentation and cytotoxicity. In the study reported here, we further ...
Highly Efficient, Near-Infrared Electrophosphorescence from a Pt–Metalloporphyrin Complex The authors acknowledge financial support from the U.S. Dept. of the Army, CECOM for the phase 14II SBIR program (contract no. 14W15P7T-06-C-T201), and Universal Display Corporation.
(WILEY-VCH Verlag, 2007-02-05)
No Abstract
The role of p53 in gemcitabine-mediated cytotoxicity and radiosensitization
(Springer-Verlag; Springer-Verlag Berlin Heidelberg, 2000-04)
Purpose : We compared the cytotoxic and radiosensitizing effects of gemcitabine (2′,2′-difluoro-2′-deoxycytidine, dFdCyd), a clinically valuable radiosensitizer, in colon cancer RKO cells which differed in their p53 status. ...
Dependence of fluorodeoxyuridine-induced cytotoxicity and megabase DNA fragment formation on S phase progression in HT29 cells
(Springer-Verlag; Springer-Verlag Berlin Heidelberg, 1996-02)
The relationship between cell cycle progression and induction of DNA double-strand breaks and cytotoxicity by exposure to fluorodeoxyuridine (FdUrd) was studied in HT29 human colon cancer cells. Fractionation of drug-treated ...
Simultaneous determination of thymine and 5-bromouracil in DNA hydrolysates using gas chromatography--mass spectrometry with selected-ion monitoring
(Elsevier, 1986)
The gas chromatographic--mass spectrometric method using selected-ion monitoring (GC--MS--SIM) described here quantitatively determines the amount of DNA thymine replacement by 5-bromouracil (BU) after exposure to ...
Analysis of bromodeoxyuridine incorporation into DNA: Comparison of gas chromatographic/mass spectrometric, CsCl gradient sedimentation, and specific radioactivity methods
(Elsevier, 1987-02-15)
A sensitive new method for the quantitation of 5-bromodeoxyuridine (BrdUrd) incorporated into DNA by GC/MS analysis of enzymatically released Thy and bromouracil (BrUra) is presented. The hydrolysis procedure was characterized ...
Interaction between gemcitabine and mitomycin-C in vitro
(Springer-Verlag; Springer-Verlag Berlin Heidelberg, 2000-01)
Both gemcitabine (2′,2′-difluorodeoxycytidine; dFdCyd) and mitomycin-C (MMC) are active against several solid malignancies. dFdCyd is an attractive agent for use in combination with drugs which damage DNA and with radiation ...
Prediction of thioguanine-induced cytotoxicity by dual-parameter flow cytometric analysis
(Springer-Verlag, 1989-10)
A method is presented for the quantitative analysis of delayed cytokinetic effects resulting from the treatment of L1210 cells with 6-thioguanine (TG). By using dual-parameter (DNA/protein) flow cytometry, we could observe ...
Enhanced cytotoxicity with methotrexate in conjunction with hypoxanthine in L1210 cells in culture
(Springer-Verlag, 1988-07)
By inhibiting dihydrofolate reductase, methotrexate (MTX) depletes cellular stores of reduced folates, resulting in the inhibition of DNA and RNA synthesis. Inhibition of RNA synthesis arrests cells in the G 1 phase of the ...
Automated integration of monolith-based protein separation with on-plate digestion for mass spectrometric analysis of esophageal adenocarcinoma human epithelial samples
(WILEY-VCH Verlag, 2006-09)
A unique approach of automating the integration of monolithic capillary HPLC-based protein separation and on-plate digestion for subsequent MALDI-MS analysis has been developed. All liquid-handling procedures were performed ...