Differential modulation of mu-opioid receptor signaling to adenylyl cyclase by regulators of G protein signaling proteins 4 or 8 and 7 in permeabilised C6 cells is Gα subtype dependent
dc.contributor.author | Talbot, Jeffery N. | en_US |
dc.contributor.author | Roman, David L. | en_US |
dc.contributor.author | Clark, Mary J. | en_US |
dc.contributor.author | Roof, Rebecca A. | en_US |
dc.contributor.author | Tesmer, John J. G. | en_US |
dc.contributor.author | Neubig, Richard R. | en_US |
dc.contributor.author | Traynor, John R. | en_US |
dc.date.accessioned | 2011-01-13T19:53:29Z | |
dc.date.available | 2011-01-13T19:53:29Z | |
dc.date.issued | 2010-02 | en_US |
dc.identifier.citation | Talbot, Jeffery N.; Roman, David L.; Clark, Mary J.; Roof, Rebecca A.; Tesmer, John J. G.; Neubig, Richard R.; Traynor, John R.; (2010). "Differential modulation of mu-opioid receptor signaling to adenylyl cyclase by regulators of G protein signaling proteins 4 or 8 and 7 in permeabilised C6 cells is Gα subtype dependent." Journal of Neurochemistry 112(4): 1026-1034. <http://hdl.handle.net/2027.42/78702> | en_US |
dc.identifier.issn | 0022-3042 | en_US |
dc.identifier.issn | 1471-4159 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/78702 | |
dc.description.abstract | J. Neurochem . (2009) 112 , 1026–1034.Regulators of G protein signaling (RGS) proteins act as GTPase-accelerating protein to negatively modulate G protein signaling and are defined by a conserved RGS domain with considerable amino acid diversity. To determine the effects of specific, purified RGS proteins on mu-opioid signaling, C6 cells stably expressing a mu-opioid receptor were rendered permeable to proteins by treatment with digitonin. Mu-opioid inhibition of forskolin-stimulated adenylyl cyclase by [ d -Ala 2 , N -Me-Phe 4 ,Gly-ol]-enkephalin (DAMGO), a mu-specific opioid peptide, remained fully intact in permeabilized cells. Purified RGS domain of RGS4 added to permeabilized cells resulted in a twofold loss in DAMGO potency but had no effect in cells expressing RGS-insensitive G proteins. The inhibitory effect of DAMGO was reduced to the same extent by purified RGS4 and RGS8. In contrast, the RGS domain of RGS7 had no effect and inhibited the action of RGS8 as a result of weak physical association with Gαi2 and minimal GTPase-accelerating protein activity in C6 cell membranes. These data suggest that differences in conserved RGS domains of specific RGS proteins contribute to differential regulation of opioid signaling to adenylyl cyclase and that a permeabilized cell model is useful for studying the effects of specific RGS proteins on aspects of G protein-coupled receptor signaling. | en_US |
dc.format.extent | 439139 bytes | |
dc.format.extent | 3106 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.subject.other | Adenylyl Cyclase | en_US |
dc.subject.other | Gα Proteins | en_US |
dc.subject.other | Mu-opioid | en_US |
dc.subject.other | Permeabilization | en_US |
dc.subject.other | Regulators of G Protein Signaling Proteins | en_US |
dc.title | Differential modulation of mu-opioid receptor signaling to adenylyl cyclase by regulators of G protein signaling proteins 4 or 8 and 7 in permeabilised C6 cells is Gα subtype dependent | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pharmacology, University of Michigan, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, University of Michigan, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Center for Chemical Genomics, University of Michigan, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Substance Abuse Research Center, University of Michigan, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationother | Department of Pharmaceutical and Biomedical Sciences, Raabe College of Pharmacy, Ohio Northern University, Ada, OH, USA | en_US |
dc.identifier.pmid | 20002516 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/78702/1/j.1471-4159.2009.06519.x.pdf | |
dc.identifier.doi | 10.1111/j.1471-4159.2009.06519.x | en_US |
dc.identifier.source | Journal of Neurochemistry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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