Distinct roles of ppGpp and DksA in Legionella pneumophila differentiation
dc.contributor.author | Dalebroux, Zachary D. | en_US |
dc.contributor.author | Yagi, Brian F. | en_US |
dc.contributor.author | Sahr, Tobias | en_US |
dc.contributor.author | Buchrieser, Carmen | en_US |
dc.contributor.author | Swanson, Michele S. | en_US |
dc.date.accessioned | 2011-01-13T19:54:25Z | |
dc.date.available | 2011-01-13T19:54:25Z | |
dc.date.issued | 2010-04 | en_US |
dc.identifier.citation | Dalebroux, Zachary D.; Yagi, Brian F.; Sahr, Tobias; Buchrieser, Carmen; Swanson, Michele S.; (2010). "Distinct roles of ppGpp and DksA in Legionella pneumophila differentiation ." Molecular Microbiology 76(1): 200-219. <http://hdl.handle.net/2027.42/78716> | en_US |
dc.identifier.issn | 0950-382X | en_US |
dc.identifier.issn | 1365-2958 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/78716 | |
dc.description.abstract | To transit between hosts, intracellular Legionella pneumophila transform into a motile, infectious, transmissive state. Here we exploit the pathogen's life cycle to examine how guanosine tetraphosphate (ppGpp) and DksA cooperate to govern bacterial differentiation. Transcriptional profiling revealed that during transmission alarmone accumulation increases the mRNA for flagellar and Type IV-secretion components, secreted host effectors and regulators, and decreases transcripts for translation, membrane modification and ATP synthesis machinery. DksA is critical for differentiation, since mutants are defective for stationary phase survival, flagellar gene activation, lysosome avoidance and macrophage cytotoxicity. The roles of ppGpp and DksA depend on the context. For macrophage transmission, ppGpp is essential, whereas DksA is dispensable, indicating that ppGpp can act autonomously. In broth, DksA promotes differentiation when ppGpp levels increase, or during fatty acid stress, as judged by flaA expression and evasion of degradation by macrophages. For flagella morphogenesis, DksA is required for basal fliA (σ 28 ) promoter activity. When alarmone levels increase, DksA cooperates with ppGpp to generate a pulse of Class II rod RNA or to amplify the Class III sigma factor and Class IV flagellin RNAs. Thus, DksA responds to the level of ppGpp and other stress signals to co-ordinate L. pneumophila differentiation. | en_US |
dc.format.extent | 217634 bytes | |
dc.format.extent | 3106 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.title | Distinct roles of ppGpp and DksA in Legionella pneumophila differentiation | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Microbiology and Immunology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA. | en_US |
dc.contributor.affiliationother | Institut Pasteur, Biologie des Bactéries Intracellulaires, Paris, France. | en_US |
dc.identifier.pmid | 20199605 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/78716/1/MMI_7094_sm_suppl_info.pdf | |
dc.identifier.doi | 10.1111/j.1365-2958.2010.07094.x | en_US |
dc.identifier.source | Molecular Microbiology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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