Maltooligosaccharides from JEG-3 Trophoblast-Like Cells Exhibit Immunoregulatory Properties
dc.contributor.author | Zhu, Aiping | en_US |
dc.contributor.author | Romero, Roberto | en_US |
dc.contributor.author | Huang, Ji-Biao | en_US |
dc.contributor.author | Clark, Andrea J. | en_US |
dc.contributor.author | Petty, Howard R. | en_US |
dc.date.accessioned | 2011-01-31T17:30:57Z | |
dc.date.available | 2012-03-05T15:30:01Z | en_US |
dc.date.issued | 2011-01 | en_US |
dc.identifier.citation | Zhu, Aiping; Romero, Roberto; Huang, Ji-Biao; Clark, Andrea; Petty, Howard R.; (2011). "Maltooligosaccharides from JEG-3 Trophoblast-Like Cells Exhibit Immunoregulatory Properties." American Journal of Reproductive Immunology 65(1): 54-64. <http://hdl.handle.net/2027.42/79123> | en_US |
dc.identifier.issn | 1046-7408 | en_US |
dc.identifier.issn | 1600-0897 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/79123 | |
dc.description.abstract | Citation Zhu A, Romero R, Huang J-B, Clark A, Petty HR. Maltooligosaccharides from JEG-3 trophoblast-like cells exhibit immunoregulatory properties. Am J Reprod Immunol 2011; 65: 54–64To better understand the immunoregulatory properties of trophoblasts, we have searched for small immunologically active carbohydrates derived from intact trophoblast-like cells.Using solid phase extraction coupled with HPLC and mass spectrometry methods, we have characterized a low molecular weight carbohydrate-rich fraction associated with JEG-3 cells. We have also tested the bioactivities of selected authentic oligosaccharides found in the oligosaccharide fraction.The most abundant components of the low molecular weight carbohydrate-rich fraction were maltotriose and maltotetraose, with detectable amounts of maltopentaose. When authentic maltooligosaccharides were tested using lymphocytes, IL-2 inhibition was observed. This activity was dependent upon the number of saccharide subunits, stereochemistry, and concentration. To further test maltooligosaccharide properties, maltopentose was attached to glass cover slips. Although spontaneous neutrophil motility was observed on unmodified and control surfaces, it was inhibited on maltooligosaccharide-derivatized surfaces.Maltooligosaccharides are associated with the trophoblast’s surface where they may exhibit immunoregulatory activities. | en_US |
dc.format.extent | 581034 bytes | |
dc.format.extent | 3106 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.subject.other | Immune Regulation | en_US |
dc.subject.other | Leukocytes | en_US |
dc.subject.other | Saccharides | en_US |
dc.title | Maltooligosaccharides from JEG-3 Trophoblast-Like Cells Exhibit Immunoregulatory Properties | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Microbiology and Immunology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Departments of Ophthalmology and Visual Sciences and Microbiology and Immunology, The University of Michigan Medical School, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationother | Perinatology Research Branch, Division of Intramural Research, Eunice Kennedy Schriver National Institute of Child Health and Human Development, NIH, Bethesda, MD and Detroit, MI, USA | en_US |
dc.contributor.affiliationother | Center for Molecular Medicine and Genetics, Wayne State University, and Hutzel Women’s Hospital at the Detroit Medical Center, Detroit, MI, USA | en_US |
dc.identifier.pmid | 20455875 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/79123/1/j.1600-0897.2010.00851.x.pdf | |
dc.identifier.doi | 10.1111/j.1600-0897.2010.00851.x | en_US |
dc.identifier.source | American Journal of Reproductive Immunology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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