Bi-Directional Calcium Signaling Between Adjacent Leukocytes and Trophoblast-Like Cells
dc.contributor.author | Elfline, Megan | en_US |
dc.contributor.author | Clark, Andrea J. | en_US |
dc.contributor.author | Petty, Howard R. | en_US |
dc.contributor.author | Romero, Roberto | en_US |
dc.date.accessioned | 2011-01-31T17:38:32Z | |
dc.date.available | 2012-01-03T20:18:47Z | en_US |
dc.date.issued | 2010-11 | en_US |
dc.identifier.citation | Elfline, Megan; Clark, Andrea; Petty, Howard R.; Romero, Roberto; (2010). "Bi-Directional Calcium Signaling Between Adjacent Leukocytes and Trophoblast-Like Cells." American Journal of Reproductive Immunology 64(5): 339-346. <http://hdl.handle.net/2027.42/79189> | en_US |
dc.identifier.issn | 1046-7408 | en_US |
dc.identifier.issn | 1600-0897 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/79189 | |
dc.description.abstract | Citation Elfline M, Clark A, Petty HR, Romero R. Bi-directional calcium signaling between adjacent leukocytes and trophoblast-like cells. Am J Reprod Immunol 2010Trophoblasts are believed to play an important role in mitigating immunological responses against the fetus. To better understand the nature of trophoblast–leukocyte interactions, we have studied signal transduction during intercellular interactions.Using a highly sensitive microfluorometric ratioing method and Ca 2+ -sensitive dyes, we measured Ca 2+ signals in trophoblast-like cell lines (JEG-3 and JAR) or in leukocytes (neutrophils and monocytes) during intercellular contact.Trophoblast cell lines exhibit Ca 2+ signals during leukocyte contact. In contrast, leukocytes cannot elicit Ca 2+ signals in non-opsonized tumour cells, suggesting that Ca 2+ signaling is not a general feature of cell–cell encounters. Similarly, leukocytes demonstrate Ca 2+ signals during contact with trophoblast cell lines. Ca 2+ signals were confirmed using three dyes and with the Ca 2+ buffer BAPTA.We suggest that leukocyte-to-trophoblast interactions lead to mutual Ca 2+ signaling events in both cell types, which may contribute to immunoregulation at the materno–fetal interface. | en_US |
dc.format.extent | 356991 bytes | |
dc.format.extent | 3106 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.subject.other | Monocytes | en_US |
dc.subject.other | Neutrophils | en_US |
dc.subject.other | Signal Transduction | en_US |
dc.title | Bi-Directional Calcium Signaling Between Adjacent Leukocytes and Trophoblast-Like Cells | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Microbiology and Immunology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Ophthalmology and Visual Sciences, The University of Michigan Medical School, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Department of Microbiology and Immunology, The University of Michigan Medical School, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationother | Perinatology Research Branch, National Institute of Child Health and Human Development, Bethesda, MD and Hutzel Hospital, Detroit, MI, USA | en_US |
dc.identifier.pmid | 20367627 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/79189/1/j.1600-0897.2010.00839.x.pdf | |
dc.identifier.doi | 10.1111/j.1600-0897.2010.00839.x | en_US |
dc.identifier.source | American Journal of Reproductive Immunology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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