Leukocyte Pyruvate Kinase Expression is Reduced in Normal Human Pregnancy but not in Pre-eclampsia
dc.contributor.author | Xu, Yi | en_US |
dc.contributor.author | Madsen-Bouterse, Sally A. | en_US |
dc.contributor.author | Romero, Roberto | en_US |
dc.contributor.author | Hassan, Sonia | en_US |
dc.contributor.author | Mittal, Pooja | en_US |
dc.contributor.author | Elfline, Megan | en_US |
dc.contributor.author | Zhu, Aiping | en_US |
dc.contributor.author | Petty, Howard R. | en_US |
dc.date.accessioned | 2011-01-31T17:42:16Z | |
dc.date.available | 2011-10-03T17:19:14Z | en_US |
dc.date.issued | 2010-08 | en_US |
dc.identifier.citation | Xu, Yi; Madsen-Bouterse, Sally A.; Romero, Roberto; Hassan, Sonia; Mittal, Pooja; Elfline, Megan; Zhu, Aiping; Petty, Howard R.; (2010). "Leukocyte Pyruvate Kinase Expression is Reduced in Normal Human Pregnancy but not in Pre-eclampsia." American Journal of Reproductive Immunology 64(2): 137-151. <http://hdl.handle.net/2027.42/79222> | en_US |
dc.identifier.issn | 1046-7408 | en_US |
dc.identifier.issn | 1600-0897 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/79222 | |
dc.description.abstract | Citation Xu Y, Madsen-Bouterse SA, Romero R, Hassan S, Mittal P, Elfline M, Zhu A, Petty HR. Leukocyte pyruvate kinase expression is reduced in normal human pregnancy but not in pre-eclampsia. Am J Reprod Immunol 2010; 64: 137–151Emerging evidence suggests that metabolism influences immune cell signaling and immunoregulation. To examine the immunoregulatory role of glycolysis in pregnancy, we evaluated the properties of pyruvate kinase in leukocytes from non-pregnant women and those with normal pregnancy and pre-eclampsia.We evaluated pyruvate kinase expression in lymphocytes and neutrophils from non-pregnant, pregnant, and pre-eclampsia patients using fluorescence microscopy and flow cytometry. Leukocyte pyruvate kinase activity and pyruvate concentrations were also evaluated. To study pyruvate’s effect on signaling, we labeled Jurkat T cells with Ca 2+ dyes and measured cell responses in the presence of agents influencing intracellular pyruvate.The expression of pyruvate kinase is reduced in lymphocytes and neutrophils from normal pregnant women in comparison with those of non-pregnant women and pre-eclampsia patients. Similarly, the activity of pyruvate kinase and the intracellular pyruvate concentration are reduced in leukocytes of normal pregnant women in comparison with non-pregnant women and women with pre-eclampsia. Using Jurkat cells as a model of leukocyte signaling, we have shown that perturbations of intracellular pyruvate influence Ca 2+ signals.Normal pregnancy is characterized by reduced pyruvate kinase expression within lymphocytes and neutrophils. We speculate that reduced pyruvate kinase expression modifies immune cell responses due to reduced pyruvate concentrations. | en_US |
dc.format.extent | 589416 bytes | |
dc.format.extent | 3106 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.subject.other | Lymphocytes | en_US |
dc.subject.other | Neutrophils | en_US |
dc.subject.other | Signaling | en_US |
dc.title | Leukocyte Pyruvate Kinase Expression is Reduced in Normal Human Pregnancy but not in Pre-eclampsia | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Microbiology and Immunology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Ophthalmology and Visual Sciences, The University of Michigan Medical School, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Department of Microbiology and Immunology, The University of Michigan Medical School, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationother | Department of Obstetrics and Gynecology, Wayne State University, Detroit, MI, USA | en_US |
dc.contributor.affiliationother | Perinatology Research Branch, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD) of NIH, Bethesda, MD and Detroit, MI, USA | en_US |
dc.contributor.affiliationother | Center of Molecular Medicine and Genetics, Hutzel Women’s Hospital at the Detroit Medical Center, Wayne State University, Detroit, MI, USA | en_US |
dc.identifier.pmid | 20560913 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/79222/1/j.1600-0897.2010.00881.x.pdf | |
dc.identifier.doi | 10.1111/j.1600-0897.2010.00881.x | en_US |
dc.identifier.source | American Journal of Reproductive Immunology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.