INFLUENCE OF INTESTINAL SURFACTANT LIKE PARTICLES ON DIFFERENTIAL ACTIVATION OF SECONDARY SIGNALING MOLECULES DURING SALMONELLA TYPHIMURIUM INFECTION
Sofi, M. Hanief; Bhatnagar, Archana; Sapra, Saveeta; Mahmood, Akhtar; Majumdar, Sidhartha
2010-05
Citation
Sofi, M. Hanief; Bhatnagar, Archana; Sapra, Saveeta; Mahmood, Akhtar; Majumdar, Sidhartha; (2010). "INFLUENCE OF INTESTINAL SURFACTANT LIKE PARTICLES ON DIFFERENTIAL ACTIVATION OF SECONDARY SIGNALING MOLECULES DURING SALMONELLA TYPHIMURIUM INFECTION." Journal of Food Safety 30(2): 455-469. <http://hdl.handle.net/2027.42/79314>
Abstract
Interaction between the enteric pathogen Salmonella typhimurium and the luminal surface of the intestine provoke an acute inflammatory response mediated in part by various inflammatory molecules. Surfactant-like particles (SLP) are known to cover the surface of the intestinal epithelium, act as lubricants and/or as a vehicle to deliver digestive enzymes to the luminal fluid. Recently we have shown that SLP plays an important protective role during microbial insult. The data suggested that SLP-induced diet prevents the damage of intestinal villi caused by S. typhimurium infection. The present study was designed to assess the role of SLP on secondary signaling molecules during S. typhimurium infection. The Peyer's patch, intraepithelial and lamina propria mononuclear cells were analyzed under various conditions for secondary signaling molecules, including calcium, inositol triphosphate and protein kinase C. SLP-induced diet along with infection showed significant variation in these secondary signaling molecules, particularly in Peyer's patches, as compared to infection group alone. In addition, the cells isolated from the infected group upon incubation in vitro with SLP also showed a considerable variation of these molecules. These results suggested an important influence of SLP on secondary signaling molecules and, in turn, could be important in overall immune modulation during infection. Salmonella are known to cause disease in humans, animals and birds worldwide. Over 1.4 million cases of salmonellosis (gastroenteritis) per year occur in the U.S.A. and other industrialized countries. Countries with poor sanitation have a much higher incidence of salmonellosis. The disruption of epithelial barrier integrity by Salmonella has pathological consequences. Intestinal epithelium secretes surfactant-like particles (SLP); these particles have the ability to cover the surface of the cell in the intestinal lumen. They are known to have ability in binding of uropathogenic Escherichia coli and in protecting the disruption of intestinal villi. The present study provides further evidence for its role in regulating secondary signaling molecules and overall immune response. SLP induced by dietary fat enhanced the resistance to intestinal infections, as it possibly decreases the colonization and translocation of S. typhimurium . The SLP and microbe interaction may thus represent a delicate balance between symbiosis and pathogenesis.Publisher
Blackwell Publishing Inc
ISSN
0149-6085 1745-4565
Other DOIs
Types
Article
Metadata
Show full item recordCollections
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.