Functional protease-activated receptors in the dorsal motor nucleus of the vagus
dc.contributor.author | Wang, H. | en_US |
dc.contributor.author | Wu, X. | en_US |
dc.contributor.author | Li, J.-Y. | en_US |
dc.contributor.author | Chai, B. -X. | en_US |
dc.contributor.author | Wang, J. | en_US |
dc.contributor.author | Mulholland, Michael W. | en_US |
dc.contributor.author | Zhang, W. | en_US |
dc.date.accessioned | 2011-01-31T17:59:09Z | |
dc.date.available | 2011-06-09T15:09:40Z | en_US |
dc.date.issued | 2010-04 | en_US |
dc.identifier.citation | Wang, H. ; Wu, X. ; Li, J.-Y. ; Chai, B.-X. ; Wang, J. ; Mulholland, M. W. ; Zhang, W. ; (2010). "Functional protease-activated receptors in the dorsal motor nucleus of the vagus." Neurogastroenterology & Motility 22(4): 431-e105. <http://hdl.handle.net/2027.42/79371> | en_US |
dc.identifier.issn | 1350-1925 | en_US |
dc.identifier.issn | 1365-2982 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/79371 | |
dc.description.abstract | Protease-activated receptors (PARs), a family member of G-protein coupled receptors, are present and functionally active in a wide variety of cells. The object of this study was to demonstrate the presence and function of PAR-1 and PAR-2 in the dorsal motor nucleus of the vagus (DMV).DMNV neurons were isolated from neonatal rat brainstems using micro-dissection and enzymatic digestion. Neurons were cultured in Neurobasal medium A containing 2% B27 supplement. Intracellular calcium concentration ([Ca 2 + ] i ) was measured using fura-2 based microspectrometry. Expression of PARs was detected by RT-PCR and immunofluorescent staining.Thrombin and PAR-1 agonist peptide activate PAR-1 with a maximum change in [Ca 2 + ] i expressed as δF/F0 of 229 ± 14% and 137 ± 7%, respectively. Trypsin and PAR-2 agonist peptide activate PAR-2 with a maximum δF/F0 change of 258 ± 12% and 242 ± 10%, respectively. Inhibition of phospholipase C (PLC) by U73312 (1 μm) decreased the maximal change in δF/F0 induced by PAR-1 activation from 140 ± 17% to 21 ± 3%, while the PAR-2-mediated maximal change in δF/F0 decreased from 185 ± 21% to 19 ± 6%. Blockade of IP3 receptor with 2APB inhibited the maximal change in δF/F0 due to PAR-1 and PAR-2 activation by 72 ± 13% and 71 ± 20% respectively. PAR-1 immnuoreactivity was present in DMV neurons. Increase in transcripts for PAR-1 and PAR-2 were detected in DMV tissues derived from IBD rats relative to control animals. Our results indicate that PAR-1 and PAR-2 are present in the DMV neurons, and their activation leads to increases in intracellular calcium via signal transduction mechanism that involves activation of PLC and the production of IP3. | en_US |
dc.format.extent | 700843 bytes | |
dc.format.extent | 3106 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.subject.other | Inflammatory Bowel Disease | en_US |
dc.subject.other | Inositol 1,4,5-trisphosphate | en_US |
dc.subject.other | Intracellular Calcium Signalling | en_US |
dc.subject.other | Phospholipase C | en_US |
dc.subject.other | Protease-activated Receptors | en_US |
dc.subject.other | The Dorsal Motor Nucleus of the Vagus | en_US |
dc.title | Functional protease-activated receptors in the dorsal motor nucleus of the vagus | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Internal Medicine and Specialties | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Surgery, University of Michigan, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationother | Department of Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China | en_US |
dc.identifier.pmid | 19719510 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/79371/1/j.1365-2982.2009.01391.x.pdf | |
dc.identifier.doi | 10.1111/j.1365-2982.2009.01391.x | en_US |
dc.identifier.source | Neurogastroenterology & Motility | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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