Adhesion and growth of dental pulp stem cells on enamel-like fluorapatite surfaces
dc.contributor.author | Liu, J. | en_US |
dc.contributor.author | Jin, Taocong | en_US |
dc.contributor.author | Chang, S-X. | en_US |
dc.contributor.author | Czajka-Jakubowska, Agata | en_US |
dc.contributor.author | Clarkson, B. H. | en_US |
dc.date.accessioned | 2011-02-02T17:58:19Z | |
dc.date.available | 2012-04-03T21:46:58Z | en_US |
dc.date.issued | 2011-03-01 | en_US |
dc.identifier.citation | Liu, J.; Jin, T. C.; Chang, S.; Czajka-Jakubowska, A.; Clarkson, B. H. (2011). "Adhesion and growth of dental pulp stem cells on enamel-like fluorapatite surfaces." Journal of Biomedical Materials Research Part A 96A(3): 528-534. <http://hdl.handle.net/2027.42/79413> | en_US |
dc.identifier.issn | 1549-3296 | en_US |
dc.identifier.issn | 1552-4965 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/79413 | |
dc.description.abstract | To study how apatite crystal alignment of an enamel-like substrate affects DPSC cellular adhesion and growth as a precursor to produce an in vitro enamel/dentin superstructure for future studies. The cells were subcultured in 10% FBS DMEM up to seven weeks on the two surfaces. Specimens were observed under SEM, counted, and analyzed using the human pathway-focused matrix and adhesion PCR array. After three days, the cell number on ordered FA surface was significantly higher than on the disordered surface. Of the 84 focused pathway genes, a total of 20 genes were either up or down regulated in the cells on ordered FA surface compared to the disordered surface. More interestingly, of the cell-matrix adhesion molecules, integrin alpha 7 and 8 (ITGA 7 and 8), integrin beta 3 and 4 (ITGB3 and 4), and the vitronectin receptor-integrin alpha V (ITGAV) and the key adhesion protein-fibronectin1 (FN1) were up-regulated. In SEM, both surfaces showed good biocompatibility and supported long term growth of DPSC cells but with functional cell-matrix interaction on the ordered FA surfaces. Significance: The enhanced cellular response of DPSC cell to the ordered FA crystal surface involves a set of delicately regulated matrix and adhesion molecules which could be manipulated by treating the cells with a dentin extract, to produce a dentin/enamel superstructure. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011. | en_US |
dc.format.extent | 550444 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Polymer and Materials Science | en_US |
dc.title | Adhesion and growth of dental pulp stem cells on enamel-like fluorapatite surfaces | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Biomedical Engineering | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Cardiology, Restorative Sciences and Endodontics, Dental School, University of Michigan, Ann Arbor, Michigan ; Department of Cariology, Restorative Sciences and Endodontics, 2310 I Dental School, University of Michigan, 1011 N. University Ave. Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationum | Department of Cardiology, Restorative Sciences and Endodontics, Dental School, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Cardiology, Restorative Sciences and Endodontics, Dental School, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Cardiology, Restorative Sciences and Endodontics, Dental School, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationother | Department of Conservative Dentistry and Periodontology, Karol Marcinkowski University of Medical Sciences, Poznan, Poland | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/79413/1/33002_ftp.pdf | |
dc.identifier.doi | 10.1002/jbm.a.33002 | en_US |
dc.identifier.source | Journal of Biomedical Materials Research Part A | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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