Raman spectroscopy monitoring of the cellular activities of a tissue-engineered ex vivo produced oral mucosal equivalent
dc.contributor.author | Lo, Wen-Liang | en_US |
dc.contributor.author | Lai, Jian-Yun | en_US |
dc.contributor.author | Feinberg, Stephen E. | en_US |
dc.contributor.author | Izumi, Kenji | en_US |
dc.contributor.author | Kao, Shou-Yen | en_US |
dc.contributor.author | Chang, Che-Shoa | en_US |
dc.contributor.author | Lin, Alan | en_US |
dc.contributor.author | Chiang, Huihua Kenny | en_US |
dc.date.accessioned | 2011-03-10T16:02:38Z | |
dc.date.accessioned | 2011-03-10T16:02:38Z | |
dc.date.available | 2012-04-03T21:46:57Z | en_US |
dc.date.issued | 2011-02 | en_US |
dc.identifier.citation | Lo, Wen-Liang; Lai, Jian-Yun; Feinberg, Stephen E.; Izumi, Kenji; Kao, Shou-Yen; Chang, Che-Shoa; Lin, Alan; Chiang, Huihua Kenny (2011). "Raman spectroscopy monitoring of the cellular activities of a tissue-engineered ex vivo produced oral mucosal equivalent." Journal of Raman Spectroscopy 42(2): 174-178. <http://hdl.handle.net/2027.42/83170> | en_US |
dc.identifier.issn | 0377-0486 | en_US |
dc.identifier.issn | 1097-4555 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/83170 | |
dc.description.abstract | To ensure quality control and assurance in tissue engineering, noninvasive, real-time and aseptic evaluation of cell-based devices is required before product release. In this study, Raman spectroscopy was applied to monitor the cellular activities of an oral mucosa equivalent (EVPOME) produced ex vivo from cultured autogenous oral keratinocytes and acellular dermis—AlloDerm. Raman spectra showed a positive correlation of the peak area ratio of amide I (1655 cm −1 )/phenylalanine (1004 cm −1 ) with a negative linear regression ( R 2 > 0.95) according to the number of cultured days, especially on the 14thand 21st day. This work demonstrates the successful application of Raman spectroscopy for quantitatively monitoring and evaluating the maturity of EVPOME. Copyright © 2010 John Wiley & Sons, Ltd. | en_US |
dc.publisher | John Wiley & Sons, Ltd. | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Analytical Chemistry and Spectroscopy | en_US |
dc.title | Raman spectroscopy monitoring of the cellular activities of a tissue-engineered ex vivo produced oral mucosal equivalent | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Physics | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Oral and Maxillofacial Surgery, University of Michigan Medical Center, Ann Arbor, MI 48109-0018, USA | en_US |
dc.contributor.affiliationother | Department of Stomatology, Taipei Veterans General Hospital, Taipei, Taiwan ; Department of Dentistry, National Yang-Ming University, Taipei, Taiwan | en_US |
dc.contributor.affiliationother | Institute of Biomedical Engineering, National Yang-Ming University, Taipei, Taiwan | en_US |
dc.contributor.affiliationother | Division of Oral Anatomy, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan | en_US |
dc.contributor.affiliationother | Department of Stomatology, Taipei Veterans General Hospital, Taipei, Taiwan ; Department of Dentistry, National Yang-Ming University, Taipei, Taiwan | en_US |
dc.contributor.affiliationother | Department of Stomatology, Taipei Veterans General Hospital, Taipei, Taiwan ; Department of Dentistry, National Yang-Ming University, Taipei, Taiwan | en_US |
dc.contributor.affiliationother | Institute of Genetic Sciences, School of Life Sciences, National Yang-Ming University, Taipei, Taiwan | en_US |
dc.contributor.affiliationother | Institute of Biomedical Engineering, National Yang-Ming University, Taipei, Taiwan ; Institute of Biomedical Engineering, National Yang-Ming University, Taipei 11221, Taiwan. | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/83170/1/2688_ftp.pdf | |
dc.identifier.doi | 10.1002/jrs.2688 | en_US |
dc.identifier.source | Journal of Raman Spectroscopy | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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