Efficient in vivo vascularization of tissue-engineering scaffolds
dc.contributor.author | Hegen, Anja | en_US |
dc.contributor.author | Blois, Anna | en_US |
dc.contributor.author | Tiron, Crina E. | en_US |
dc.contributor.author | Hellesøy, Monica | en_US |
dc.contributor.author | Micklem, David R. | en_US |
dc.contributor.author | Nör, Jacques E. | en_US |
dc.contributor.author | Akslen, Lars A. | en_US |
dc.contributor.author | Lorens, James B. | en_US |
dc.date.accessioned | 2011-04-07T18:52:20Z | |
dc.date.accessioned | 2011-04-07T18:52:20Z | |
dc.date.available | 2012-05-14T20:40:08Z | en_US |
dc.date.issued | 2011-04 | en_US |
dc.identifier.citation | Hegen, Anja; Blois, Anna; Tiron, Crina E.; Hellesøy, Monica; Micklem, David R.; Nör, Jacques E.; Akslen, Lars A.; Lorens, James B. (2011). "Efficient in vivo vascularization of tissue-engineering scaffolds." Journal of Tissue Engineering and Regenerative Medicine 5(4): e52-e62. <http://hdl.handle.net/2027.42/83466> | en_US |
dc.identifier.issn | 1932-6254 | en_US |
dc.identifier.issn | 1932-7005 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/83466 | |
dc.description.abstract | The success of tissue engineering depends on the rapid and efficient formation of a functional blood vasculature. Adult blood vessels comprise endothelial cells and perivascular mural cells that assemble into patent tubules ensheathed by a basement membrane during angiogenesis. Using individual vessel components, we characterized intra-scaffold microvessel self-assembly efficiency in a physiological in vivo tissue engineering implant context. Primary human microvascular endothelial and vascular smooth muscle cells were seeded at different ratios in poly- L -lactic acid (PLLA) scaffolds enriched with basement membrane proteins (Matrigel) and implanted subcutaneously into immunocompromised mice. Temporal intra-scaffold microvessel formation, anastomosis and perfusion were monitored by immunohistochemical, flow cytometric and in vivo multiphoton fluorescence microscopy analysis. Vascularization in the tissue-engineering context was strongly enhanced in implants seeded with a complete complement of blood vessel components: human microvascular endothelial and vascular smooth muscle cells in vivo assembled a patent microvasculature within Matrigel-enriched PLLA scaffolds that anastomosed with the host circulation during the first week of implantation. Multiphoton fluorescence angiographic analysis of the intra-scaffold microcirculation showed a uniform, branched microvascular network. 3D image reconstruction analysis of human pulmonary artery smooth muscle cell (hPASMC) distribution within vascularized implants was non-random and displayed a preferential perivascular localization. Hence, efficient microvessel self-assembly, anastomosis and establishment of a functional microvasculture in the native hypoxic in vivo tissue engineering context is promoted by providing a complete set of vascular components. Copyright © 2010 John Wiley & Sons, Ltd. | en_US |
dc.publisher | John Wiley & Sons, Ltd. | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Medicine | en_US |
dc.title | Efficient in vivo vascularization of tissue-engineering scaffolds | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Medicine (General) | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | School of Dentistry and Department of Otolaryngology, University of Michigan, Ann Arbor, MI 48109-1078, USA | en_US |
dc.contributor.affiliationother | Department of Biomedicine, University of Bergen, N-5009 Bergen, Norway ; These authors contributed equally to this study. | en_US |
dc.contributor.affiliationother | Department of Biomedicine, University of Bergen, N-5009 Bergen, Norway | en_US |
dc.contributor.affiliationother | Department of Biomedicine, University of Bergen, N-5009 Bergen, Norway | en_US |
dc.contributor.affiliationother | Department of Biomedicine, University of Bergen, N-5009 Bergen, Norway | en_US |
dc.contributor.affiliationother | Department of Biomedicine, University of Bergen, N-5009 Bergen, Norway | en_US |
dc.contributor.affiliationother | The Gade Institute, Section for Pathology, University of Bergen, N-5009 Bergen, Norway ; Department of Pathology, Haukeland University Hospital, N-5021 Bergen, Norway | en_US |
dc.contributor.affiliationother | Department of Biomedicine, University of Bergen, N-5009 Bergen, Norway ; Department of Biomedicine, University of Bergen, Jonas Lies Vei 91, N-5009 Bergen, Norway. | en_US |
dc.identifier.pmid | 20865694 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/83466/1/336_ftp.pdf | |
dc.identifier.doi | 10.1002/term.336 | en_US |
dc.identifier.source | Journal of Tissue Engineering and Regenerative Medicine | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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