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Improved detection of nicotinamide adenine dinucleotide phosphate oscillations within human neutrophils
dc.contributor.authorClark, Andrea J.en_US
dc.contributor.authorRomero, Robertoen_US
dc.contributor.authorPetty, Howard R.en_US
dc.date.accessioned2011-06-10T14:21:07Z
dc.date.available2011-12-02T15:41:52Zen_US
dc.date.issued2010-10en_US
dc.identifier.citationClark, Andrea J.; Romero, Roberto; Petty, Howard R. (2010). "Improved detection of nicotinamide adenine dinucleotide phosphate oscillations within human neutrophils." Cytometry Part A 77A(10): 976-982. <http://hdl.handle.net/2027.42/84392>en_US
dc.identifier.issn1552-4922en_US
dc.identifier.issn1552-4930en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/84392
dc.description.abstractKinetic studies of nicotinamide adenine dinucleotide phosphate autofluorescence have been conducted in adherent neutrophils using an improved microscopic photometry system incorporating low noise excitation and detection systems. Dynamic autofluorescence oscillations were found with periods ranging from ∼4 min to ∼10 s. The largest portion of the population of oscillating neutrophils (32%) had periods near 2 min. The next largest group at 25% exhibited periods of 1 min or less. These oscillations could not be accounted for by instrument artifacts, cell shape changes away from the focal plane, or other factors. They disappeared when detergent was added to oscillating cells. Higher-frequency oscillations disappeared as cells changed shape, indicating a correlation between these two processes. This approach provides a reliable method to monitor this cellular property. © 2010 International Society for Advancement of Cytometryen_US
dc.publisherWiley Subscription Services, Inc., A Wiley Companyen_US
dc.subject.otherLife and Medical Sciencesen_US
dc.subject.otherCell & Developmental Biologyen_US
dc.titleImproved detection of nicotinamide adenine dinucleotide phosphate oscillations within human neutrophilsen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Ophthalmology and Visual Sciences, The University of Michigan Medical School, Ann Arbor, MIen_US
dc.contributor.affiliationumDepartment of Ophthalmology and Visual Sciences, The University of Michigan Medical School, Ann Arbor, MI ; Department of Microbiology and Immunology, The University of Michigan Medical School, Ann Arbor, MI ; Department of Ophthalmology and Visual Sciences, 1000 Wall Street, The University of Michigan Medical School, Ann Arbor, MI 48105, USAen_US
dc.contributor.affiliationotherPerinatology Research Branch, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD) of NIH, Bethesda, Maryland, and Detroit, Michigan ; Center of Molecular Medicine and Genetics, Wayne State University, Detroit, Michigan ; Hutzel Women's Hospital at the Detroit Medical Center, Detroit, Michiganen_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/84392/1/20961_ftp.pdf
dc.identifier.doi10.1002/cyto.a.20961en_US
dc.identifier.sourceCytometry Part Aen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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