John Cunningham virus T-antigen expression in anal carcinoma
dc.contributor.author | Ramamoorthy, Sonia | en_US |
dc.contributor.author | Devaraj, Bikash | en_US |
dc.contributor.author | Miyai, Katsumi | en_US |
dc.contributor.author | Luo, Linda | en_US |
dc.contributor.author | Liu, Yu-Tsueng | en_US |
dc.contributor.author | Boland, C. Richard | en_US |
dc.contributor.author | Goel, Ajay | en_US |
dc.contributor.author | Carethers, John M. | en_US |
dc.date.accessioned | 2011-06-10T14:21:28Z | |
dc.date.available | 2012-07-12T17:42:23Z | en_US |
dc.date.issued | 2011-06-01 | en_US |
dc.identifier.citation | Ramamoorthy, Sonia; Devaraj, Bikash; Miyai, Katsumi; Luo, Linda; Liu, Yu-Tsueng; Boland, C. Richard; Goel, Ajay; Carethers, John M. (2011). "John Cunningham virus T-antigen expression in anal carcinoma." Cancer 117(11): 2379-2385. <http://hdl.handle.net/2027.42/84407> | en_US |
dc.identifier.issn | 0008-543X | en_US |
dc.identifier.issn | 1097-0142 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/84407 | |
dc.description.abstract | BACKGROUND: Anal carcinoma is thought to be driven by human papillomavirus (HPV) infection through interrupting function of cell regulatory proteins such as p53 and pRb. John Cunningham virus (JCV) expresses a T-antigen that causes malignant transformation through development of aneuploidy and interaction with some of the same regulatory proteins as HPV. JCV T-antigen is present in brain, gastric, and colon malignancies, but has not been evaluated in anal cancers. The authors examined a cohort of anal cancers for JCV T-antigen and correlated this with clinicopathologic data. METHODS: Archived anal carcinomas were analyzed for JCV T-antigen expression. DNA from tumor and normal tissue was sequenced for JCV with viral copies determined by quantitative polymerase chain reaction and Southern blotting. HPV and microsatellite instability (MSI) status was correlated with JCV T-antigen expression. RESULTS: Of 21 cases of anal cancer (mean age 49 years, 38% female), 12 (57%) were in human immunodeficiency virus (HIV)-positive individuals. All 21 cancers expressed JCV T-antigen, including 9 HPV-negative specimens. More JCV copies were present in cancer versus surrounding normal tissue (mean 32.54 copies/μg DNA vs 2.98 copies/μg DNA, P = .0267). There was no correlation between disease stage and viral copies, nor between viral copies and HIV-positive or -negative status (28.7 vs 36.34 copies/μg DNA, respectively, P = .7804). In subset analysis, no association was found between JCV T-antigen expression and HPV or MSI status. CONCLUSIONS: Anal carcinomas uniformly express JCV T-antigen and contain more viral copies compared with surrounding normal tissue. JCV and its T-antigen oncogenic protein, presumably through interruption of cell regulatory proteins, may play a role in anal cancer pathogenesis. Cancer 2011. © 2010 American Cancer Society. | en_US |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Cancer Research, Oncology and Pathology | en_US |
dc.title | John Cunningham virus T-antigen expression in anal carcinoma | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Oncology and Hematology | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Moores Comprehensive Cancer Center, University of California, San Diego, California ; Department of Medicine, University of California, San Diego, California ; Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan ; Fax: (734) 232-3838 ; Department of Internal Medicine, University of Michigan, 1500 E. Medical Center Drive, SPC 5368, Ann Arbor, MI 48109-5368 | en_US |
dc.contributor.affiliationother | Department of Surgery, University of California, San Diego, California ; Moores Comprehensive Cancer Center, University of California, San Diego, California | en_US |
dc.contributor.affiliationother | Department of Surgery, University of California, San Diego, California | en_US |
dc.contributor.affiliationother | Department of Pathology, University of California, San Diego, California | en_US |
dc.contributor.affiliationother | Department of Surgery, University of California, San Diego, California | en_US |
dc.contributor.affiliationother | Moores Comprehensive Cancer Center, University of California, San Diego, California ; Department of Medicine, University of California, San Diego, California | en_US |
dc.contributor.affiliationother | Gastrointestinal Cancer Research Laboratory, Baylor University Medical Center, Dallas, Texas | en_US |
dc.contributor.affiliationother | Gastrointestinal Cancer Research Laboratory, Baylor University Medical Center, Dallas, Texas | en_US |
dc.identifier.pmid | 24048785 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/84407/1/25793_ftp.pdf | |
dc.identifier.doi | 10.1002/cncr.25793 | en_US |
dc.identifier.source | Cancer | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.