Bioengineered Three-Dimensional Physiological Model of Colonic Longitudinal Smooth Muscle In Vitro
dc.contributor.author | Raghavan, Shreya | en_US |
dc.contributor.author | Lam, Mai T. | en_US |
dc.contributor.author | Foster, Lesley L. | en_US |
dc.contributor.author | Gilmont, Robert R. | en_US |
dc.contributor.author | Somara, Sita | en_US |
dc.contributor.author | Takayama, Shuichi | en_US |
dc.contributor.author | Bitar, Khalil N. | en_US |
dc.date.accessioned | 2011-06-17T20:26:37Z | |
dc.date.available | 2011-06-17T20:26:37Z | |
dc.date.issued | 2009 | en_US |
dc.identifier.citation | Raghavan, Shreya; Lam, Mai T.; Foster, Lesley L.; Gilmont, Robert R.; Somara, Sita; Takayama, Shuichi; Bitar, Khalil N. (2009/12/15). "Bioengineered Three-Dimensional Physiological Model of Colonic Longitudinal Smooth Muscle In Vitro." Tissue Engineering Part C: Methods, 16(5): 999-1009 <http://hdl.handle.net/2027.42/85093> | en_US |
dc.identifier.issn | 1937-3384 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/85093 | |
dc.description.abstract | Background: The objective of this study was to develop a physiological model of longitudinal smooth muscle tissue from isolated longitudinal smooth muscle cells arranged in the longitudinal axis. Methods: Longitudinal smooth muscle cells from rabbit sigmoid colon were isolated and expanded in culture. Cells were seeded at high densities onto laminin-coated Sylgard surfaces with defined wavy microtopographies. A highly aligned cell sheet was formed, to which addition of fibrin resulted in delamination. Results: (1) Acetylcholine (ACh) induced a dose-dependent, rapid, and sustained force generation. (2) Absence of extracellular calcium attenuated the magnitude and sustainability of ACh-induced force by 50% and 60%, respectively. (3) Vasoactive intestinal peptide also attenuated the magnitude and sustainability of ACh-induced force by 40% and 60%, respectively. These data were similar to force generated by longitudinal tissue. (4) Bioengineered constructs also maintained smooth muscle phenotype and calcium-dependence characteristics. Summary: This is a novel physiologically relevant in vitro three-dimensional model of colonic longitudinal smooth muscle tissue. Bioengineered three-dimensional longitudinal smooth muscle presents the ability to generate force, and respond to contractile agonists and relaxant peptides similar to native longitudinal tissue. This model has potential applications to investigate the underlying pathophysiology of dysfunctional colonic motility. It also presents as a readily implantable band-aid colonic longitudinal muscle tissue. | en_US |
dc.publisher | Mary Ann Liebert, Inc., publishers | en_US |
dc.title | Bioengineered Three-Dimensional Physiological Model of Colonic Longitudinal Smooth Muscle In Vitro | en_US |
dc.type | Article | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.identifier.pmid | 20001822 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/85093/1/ten_tec_2009_0394.pdf | |
dc.identifier.doi | 10.1089/ten.tec.2009.0394 | en_US |
dc.identifier.source | Tissue Engineering Part C: Methods | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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