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Induction of Human Blood Group A Antigen Expression on Mouse Cells, Using Lentiviral Gene Transduction

dc.contributor.authorFan, Xiaohuen_US
dc.contributor.authorLang, Hailien_US
dc.contributor.authorZhou, Xianpeien_US
dc.contributor.authorZhang, Lien_US
dc.contributor.authorYin, Rongen_US
dc.contributor.authorMaciejko, Jessicaen_US
dc.contributor.authorGiannitsos, Vasilikien_US
dc.contributor.authorMotyka, Bruceen_US
dc.contributor.authorMedin, Jeffrey A.en_US
dc.contributor.authorPlatt, Jeffrey L.en_US
dc.contributor.authorWest, Lori J.en_US
dc.date.accessioned2011-06-17T20:27:31Z
dc.date.available2011-06-17T20:27:31Z
dc.date.issued2010en_US
dc.identifier.citationFan, Xiaohu; Lang, Haili; Zhou, Xianpei; Zhang, Li; Yin, Rong; Maciejko, Jessica; Giannitsos, Vasiliki; Motyka, Bruce; Medin, Jeffrey A.; Platt, Jeffrey L.; West, Lori J. (2010/02/17). "Induction of Human Blood Group A Antigen Expression on Mouse Cells, Using Lentiviral Gene Transduction." Human Gene Therapy, 21(7): 877-890 <http://hdl.handle.net/2027.42/85136>en_US
dc.identifier.issn1043-0342en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/85136
dc.description.abstractAbstract The ABO histo-blood group system is the most important antigen system in transplantation medicine, yet no small animal model of the ABO system exists. To determine the feasibility of developing a murine model, we previously subcloned the human α-1,2-fucosyltransferase (H-transferase, EC 2.4.1.69) cDNA and the human α-1,3-N-acetylgalactosaminyltransferase (A-transferase, EC 2.4.1.40) cDNA into lentiviral vectors to study their ability to induce human histo-blood group A antigen expression on mouse cells. Herein we investigated the optimal conditions for human A and H antigen expression in murine cells. We determined that transduction of a bicistronic lentiviral vector (LvEF1-AH-trs) resulted in the expression of A antigen in a mouse endothelial cell line. We also studied the in vivo utility of this vector to induce human A antigen expression in mouse liver. After intrahepatic injection of LvEF1-AH-trs, A antigen expression was observed on hepatocytes as detected by immunohistochemistry and real-time RT-PCR. In human group A erythrocyte-sensitized mice, A antigen expression in the liver was associated with tissue damage, and deposition of antibody and complement. These results suggest that this gene transfer strategy can be used to simulate the human ABO blood group system in a murine model. This model will facilitate progress in the development of interventions for ABO-incompatible transplantation and transfusion scenarios, which are difficult to develop in clinical or large animal settings.en_US
dc.publisherMary Ann Liebert, Inc., publishersen_US
dc.titleInduction of Human Blood Group A Antigen Expression on Mouse Cells, Using Lentiviral Gene Transductionen_US
dc.typeArticleen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.identifier.pmid20163247en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/85136/1/hum_2008_089.pdf
dc.identifier.doi10.1089/hum.2008.089en_US
dc.identifier.sourceHuman Gene Therapyen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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