Folate‐targeted nanoparticles show efficacy in the treatment of inflammatory arthritis
dc.contributor.author | Thomas, Thommey P. | en_US |
dc.contributor.author | Goonewardena, Sascha N. | en_US |
dc.contributor.author | Majoros, Istvan J. | en_US |
dc.contributor.author | Kotlyar, Alina | en_US |
dc.contributor.author | Cao, Zhengyi | en_US |
dc.contributor.author | Leroueil, Pascale Raymonde | en_US |
dc.contributor.author | Baker, James R. Jr. | en_US |
dc.date.accessioned | 2011-11-10T15:34:20Z | |
dc.date.available | 2012-11-02T18:56:40Z | en_US |
dc.date.issued | 2011-09 | en_US |
dc.identifier.citation | Thomas, Thommey P.; Goonewardena, Sascha N.; Majoros, Istvan J.; Kotlyar, Alina; Cao, Zhengyi; Leroueil, Pascale R.; Baker, James R. (2011). "Folate‐targeted nanoparticles show efficacy in the treatment of inflammatory arthritis." Arthritis & Rheumatism 63(9): 2671-2680. <http://hdl.handle.net/2027.42/86938> | en_US |
dc.identifier.issn | 0004-3591 | en_US |
dc.identifier.issn | 1529-0131 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/86938 | |
dc.description.abstract | Objective To investigate the uptake of a poly(amidoamine) dendrimer (generation 5 [G5]) nanoparticle covalently conjugated to polyvalent folic acid (FA) as the targeting ligand into macrophages, and to investigate the activity of an FA‐ and methotrexate (MTX)–conjugated dendrimer (G5‐FA‐MTX) as a therapeutic for the inflammatory disease of arthritis. Methods In vitro studies were performed in macrophage cell lines and in isolated mouse macrophages to check the cellular uptake of fluorescence‐tagged G5‐FA nanoparticles, using flow cytometry and confocal microscopy. In vivo studies were conducted in a rat model of collagen‐induced arthritis to evaluate the therapeutic potential of G5‐FA‐MTX. Results Folate‐targeted dendrimer bound and internalized in a receptor‐specific manner into both folate receptor β–expressing macrophage cell lines and primary mouse macrophages. The conjugate G5‐FA‐MTX acted as a potent antiinflammatory agent and reduced arthritis‐induced parameters of inflammation such as ankle swelling, paw volume, cartilage damage, bone resorption, and body weight decrease. Conclusion The use of folate‐targeted nanoparticles to specifically target MTX into macrophages may provide an effective clinical approach for antiinflammatory therapy in rheumatoid arthritis. | en_US |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.title | Folate‐targeted nanoparticles show efficacy in the treatment of inflammatory arthritis | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Geriatrics | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | University of Michigan, Ann Arbor | en_US |
dc.contributor.affiliationum | Michigan Nanotechnology Institute for Medicine and Biological Sciences, Department of Internal Medicine, Division of Allergy, 1150 West Medical Center Drive, Room 9220C MSRB III, University of Michigan, Ann Arbor, MI 48109 | en_US |
dc.identifier.pmid | 21618461 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/86938/1/30459_ftp.pdf | |
dc.identifier.doi | 10.1002/art.30459 | en_US |
dc.identifier.source | Arthritis & Rheumatism | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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