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Infiltrating lymphocytes and human papillomavirus‐16–associated oropharyngeal cancer

dc.contributor.authorWansom, Derricken_US
dc.contributor.authorLight, Emilyen_US
dc.contributor.authorThomas, Dafydd G.en_US
dc.contributor.authorWorden, Francis P.en_US
dc.contributor.authorPrince, Mark E. P.en_US
dc.contributor.authorUrba, Susan G.en_US
dc.contributor.authorChepeha, Douglas B.en_US
dc.contributor.authorKumar, Bhavnaen_US
dc.contributor.authorCordell, Kitrina G.en_US
dc.contributor.authorEisbruch, Avrahamen_US
dc.contributor.authorTaylor, Jeremyen_US
dc.contributor.authorMoyer, Jeffrey S.en_US
dc.contributor.authorBradford, Carol R.en_US
dc.contributor.authorD'Silva, Nisha J.en_US
dc.contributor.authorCarey, Thomasen_US
dc.contributor.authorMcHugh, Jonathan B.en_US
dc.contributor.authorWolf, Gregory T.en_US
dc.date.accessioned2012-01-05T22:04:49Z
dc.date.available2013-03-04T15:29:54Zen_US
dc.date.issued2012-01en_US
dc.identifier.citationWansom, Derrick; Light, Emily; Thomas, Dafydd; Worden, Francis; Prince, Mark; Urba, Susan; Chepeha, Douglas; Kumar, Bhavna; Cordell, Kitrina; Eisbruch, Avraham; Taylor, Jeremy; Moyer, Jeffrey; Bradford, Carol; D'Silva, Nisha; Carey, Thomas; McHugh, Jonathan; Wolf, Gregory (2012). "Infiltrating lymphocytes and human papillomavirus‐16–associated oropharyngeal cancer ." The Laryngoscope 122(1): 121-127. <http://hdl.handle.net/2027.42/89454>en_US
dc.identifier.issn0023-852Xen_US
dc.identifier.issn1531-4995en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/89454
dc.description.abstractObjectives/Hypothesis: Human papillomavirus‐16 (HPV‐16)–associated carcinoma of the oropharynx has a favorable prognosis. Such patients have elevated CD8+ T‐lymphocyte levels that correlate with response to chemotherapy and survival. Tumor‐infiltrating lymphocyte (TIL) subpopulations were assessed in pretreatment biopsies from a prospective patient cohort to determine if TIL subsets differed by HPV status, clinical factors, or patient outcome or correlated with peripheral blood T‐cell levels. Study Design: Retrospective immunological correlative study of patients entered in a prospective Phase 2 clinical trial. Methods: Measured were CD8, CD4, CD68, and Treg (FoxP3) lymphocytes by immunohistochemistry in a tissue microarray created from patients (n = 46) with advanced oropharyngeal cancer. Correlations with peripheral blood levels, HPV status, expression of epidermal growth factor receptor (EGFR), clinical tumor, and patient characteristics and outcome were determined. Median follow‐up was 6.6 years. Results: HPV‐16–positive patients had improved survival ( P = .016). Degree of T‐cell infiltration did not differ by HPV status but was significantly related to disease‐specific survival (DSS) and overall survival (OS). Even after adjusting for HPV status, we found that CD8, FoxP3, and total T cells were significantly associated with DSS ( P = .0236, P = .0040, and P = .0197, respectively) and OS ( P = .0137, P = .0158, and P = .0115, respectively). Less T‐cell infiltration ( P = .0130) and CD4 cells in particular ( P = .0792) were associated with higher EGFR expression. Conclusions: Improved outcomes are associated with increased TILs independent of HPV status and suggest the local immune response may be more related to factors such as tumor size, EGFR expression, or performance status than HPV status. Further study of larger numbers of patients and infiltrates combined with functional analysis of individual subsets may be necessary to detect significant differences in local immunity in HPV‐16–related cancers.Laryngoscope, 122:121–127, 2012en_US
dc.publisherWiley Subscription Services, Inc., A Wiley Companyen_US
dc.subject.otherHuman Papillomavirusen_US
dc.subject.otherT Cell Infiltratesen_US
dc.subject.otherOropharyngeal Canceren_US
dc.subject.otherCellular Immunityen_US
dc.subject.otherLevel of Evidence : 1ben_US
dc.titleInfiltrating lymphocytes and human papillomavirus‐16–associated oropharyngeal canceren_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelOtolaryngologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Otolaryngology–Head and Neck Surgery; University of Michigan, Ann Arbor, Michigan, U.S.Aen_US
dc.contributor.affiliationumDepartment of Pathology; University of Michigan, Ann Arbor, Michigan, U.S.Aen_US
dc.contributor.affiliationumDepartment of Biostatistics; University of Michigan, Ann Arbor, Michigan, U.S.Aen_US
dc.contributor.affiliationumDepartment of Medicine; University of Michigan, Ann Arbor, Michigan, U.S.Aen_US
dc.contributor.affiliationumDepartment of Radiation Oncology; University of Michigan, Ann Arbor, Michigan, U.S.Aen_US
dc.contributor.affiliationumDepartment of Periodontics and Oral Medicine; University of Michigan, Ann Arbor, Michigan, U.S.Aen_US
dc.contributor.affiliationumDepartment of Otolaryngology–Head and Neck Surgery, University of Michigan Hospitals, 1903 Taubman Bldg, Box 5312, Ann Arbor, MI 48109en_US
dc.identifier.pmid22183632en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/89454/1/22133_ftp.pdf
dc.identifier.doi10.1002/lary.22133en_US
dc.identifier.sourceThe Laryngoscopeen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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