Validation of a new multiplex assay against individual immunoassays for the quantification of reproductive, stress, and energetic metabolism biomarkers in urine specimens
dc.contributor.author | Salvante, Katrina G. | en_US |
dc.contributor.author | Brindle, Eleanor | en_US |
dc.contributor.author | Mcconnell, Daniel | en_US |
dc.contributor.author | O'connor, Kathleen | en_US |
dc.contributor.author | Nepomnaschy, Pablo A. | en_US |
dc.date.accessioned | 2012-01-05T22:06:36Z | |
dc.date.available | 2013-03-04T15:29:55Z | en_US |
dc.date.issued | 2012-01 | en_US |
dc.identifier.citation | Salvante, Katrina G.; Brindle, Eleanor; Mcconnell, Daniel; O'connor, Kathleen; Nepomnaschy, Pablo A. (2012). "Validation of a new multiplex assay against individual immunoassays for the quantification of reproductive, stress, and energetic metabolism biomarkers in urine specimens." American Journal of Human Biology 24(1): 81-86. <http://hdl.handle.net/2027.42/89528> | en_US |
dc.identifier.issn | 1042-0533 | en_US |
dc.identifier.issn | 1520-6300 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/89528 | |
dc.description.abstract | Abstract: Measuring multiple hormones simultaneously in a single assay saves sample volume, labor, time, reagents, money, and consumables. Thus, multiplex arrays represent a faster, more economically and ecologically sound alternative to singleton assays. Objectives: To validate a new, commercially available multiplex female array produced by Quansys Biosciences against individual immunoassays for the quantification of six hormones in urine samples from women in different reproductive stages. Methods: Urine samples were analyzed using the new Quansys multiplex female hormone array and compared with well‐established individual immunoassays for adiponectin, free cortisol, c‐peptide, estrone‐3‐glucuronide (E 1 G), follicle stimulating hormone beta‐subunit (FSH‐beta), and human chorionic gonadotropin beta‐subunit (hCG‐beta). Correlations between assays were assessed using Pearson correlation, linear regression and Bland–Altman analysis. The temporal profiles of free cortisol, E1G, FSH‐beta, and hCG‐beta were also compared. Results: The multiplex array was highly correlated with the individual immunoassays for five of the tested hormones (Pearson's correlation coefficient ≥0.75), and yielded temporal patterns of hormone profiles consistent with the individual immunoassays for free cortisol, E1G, FSH‐beta, and hCG‐beta. Conclusions: The Quansys multiplex female hormone array is a valid alternative method to individual immunoassays for the quantification of stress, reproductive and energetic hormones and metabolites in human urine samples and can be used to examine the dynamic interactions between these hormones. Am. J. Hum. Biol., 2012. © 2011 Wiley Periodicals, Inc. | en_US |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.title | Validation of a new multiplex assay against individual immunoassays for the quantification of reproductive, stress, and energetic metabolism biomarkers in urine specimens | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Medicine (General) | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Reproductive Sciences Program, The University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Department of Epidemiology, The University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationother | Faculty of Health Sciences, Simon Fraser University, Burnaby, British Columbia, Canada V5A 1S6 | en_US |
dc.contributor.affiliationother | Center for Studies in Demography and Ecology, University of Washington, Seattle, Washington 98195 | en_US |
dc.contributor.affiliationother | Department of Anthropology, University of Washington, Seattle, Washington 98195 | en_US |
dc.contributor.affiliationother | Human Evolutionary Studies Program, Simon Fraser University, Burnaby, British Columbia, Canada V5A 1S6 | en_US |
dc.contributor.affiliationother | Faculty of Health Sciences, Simon Fraser University, Blusson Hall 9417, 8888 University Drive, Burnaby, British Columbia, V5A 1S6, Canada | en_US |
dc.identifier.pmid | 22121074 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/89528/1/21229_ftp.pdf | |
dc.identifier.doi | 10.1002/ajhb.21229 | en_US |
dc.identifier.source | American Journal of Human Biology | en_US |
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dc.identifier.citedreference | Brindle E, Miller RC, Shofer JB, Klein NA, Soules MR, O'Connor KA. 2006. Urinary beta‐luteinizing hormones and beta‐follicle stimulating hormone immunoenzymometric assays for population research. Clin Biochem 39: 1071 – 1079. | en_US |
dc.identifier.citedreference | Nepomnaschy PA, Lee TCK, Zeng L, Dean CB. Using first morning cortisol levels to compare physiologic stress levels between individuals: Ranking methods and sample sizes. Submitted to Am J Hum Biol (under review). | en_US |
dc.identifier.citedreference | Nepomnaschy PA, Welch KB, McConnell DS, Low BS, Strassmann BI, England BG. 2006. Cortisol levels and very early pregnancy loss in humans. Proc Natl Acad Sci USA 103: 3938 – 3942. | en_US |
dc.identifier.citedreference | Nepomnaschy PA, Welch K, McConnell D, Strassmann BI, England BG. 2004. Stress and female reproductive function: a study of daily variations in cortisol, gonadotrophins, and gonadal steroids in a rural Mayan population. Am J Hum Biol 16: 523 – 532. | en_US |
dc.identifier.citedreference | O'Connor KA, Brindle E, Shofer JB, Miller RC, Klein NA, Soules MR, Campbell KL, Mar C, Handcock MS. 2004. Statistical correction for non‐parallelism in a urinary enzyme immunoassay. J Immunoassay Immunochem 25: 259 – 278. | en_US |
dc.identifier.citedreference | Santoro N, Crawford SL, Allsworth JE, Gold EB, Greendale GA, Korenman S, Lasley BL, McConnell D, McGaffigan P, Midgely R, Schocken M, Sowers M, Weiss G. 2003. Assessing menstrual cycles with urinary hormone assays. Am J Phys Endocrinol Metab 284: E521 – E530. | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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