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Quantification of genes and gene transcripts for microbial perchlorate reduction in fixed‐bed bioreactors
dc.contributor.authorDe Long, S.K.en_US
dc.contributor.authorLi, X.en_US
dc.contributor.authorBae, S.en_US
dc.contributor.authorBrown, J.C.en_US
dc.contributor.authorRaskin, L.en_US
dc.contributor.authorKinney, K.A.en_US
dc.contributor.authorKirisits, M.J.en_US
dc.date.accessioned2012-03-16T15:59:32Z
dc.date.available2013-05-01T17:24:41Zen_US
dc.date.issued2012-03en_US
dc.identifier.citationDe Long, S.K.; Li, X.; Bae, S.; Brown, J.C.; Raskin, L.; Kinney, K.A.; Kirisits, M.J. (2012). "Quantification of genes and gene transcripts for microbial perchlorate reduction in fixed‐bed bioreactors." Journal of Applied Microbiology 112(3). <http://hdl.handle.net/2027.42/90307>en_US
dc.identifier.issn1364-5072en_US
dc.identifier.issn1365-2672en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/90307
dc.description.abstractAims:  Optimization of full‐scale, biological perchlorate treatment processes for drinking water would benefit from knowledge of the location and quantity of perchlorate‐reducing bacteria (PRB) and expression of perchlorate‐related genes in bioreactors. The aim of this study was to quantify perchlorate removal and perchlorate‐related genes ( pcrA and cld ) and their transcripts in bioreactors and to determine whether these genes or transcripts could serve as useful biomarkers for perchlorate treatment processes. Methods and Results:  Quantitative PCR (qPCR) assays targeting pcrA and cld were applied to two pilot‐scale, fixed‐bed bioreactors treating perchlorate‐contaminated groundwater. pcrA and cld genes per microgram of DNA were two‐ to threefold higher and three‐ to fourfold higher, respectively, in the bioreactor showing superior perchlorate‐removal performance. In a laboratory‐scale bioreactor, quantities of pcrA and cld genes and transcripts were compared under two distinct performance conditions ( c.  60 and 20% perchlorate removal) for a 5‐min empty bed contact time. cld genes per microgram of DNA were approximately threefold higher and cld transcripts per microgram of RNA were approximately sixfold higher under the higher perchlorate‐removal condition. No differences in pcrA genes or transcripts per microgram of DNA or RNA, respectively, were detected between the c.  60 and 20% perchlorate‐removal conditions, possibly because these assays did not accurately quantify pcrA genes and transcripts in the mixed culture present. Conclusions:  Quantities of cld genes and transcripts per microgram of DNA and RNA, respectively, were found to be higher when perchlorate removal was higher. However, quantities of pcrA and cld genes or transcripts were not found to directly correlate with perchlorate‐removal rates. Significance and Impact of the Study:  To our knowledge, this study represents the first application of qPCR assays to quantify perchlorate‐related genes and transcripts in continuous‐flow bioreactors. The results indicate that cld gene and transcript quantities can provide insights regarding the quantity, location and gene expression of PRB in bioreactors.en_US
dc.publisherBlackwell Publishing Ltden_US
dc.publisherWiley Periodicals, Inc.en_US
dc.subject.otherBiological Drinking Water Treatmenten_US
dc.subject.otherPerchlorate‐Reducing Bacteriaen_US
dc.subject.otherQuantitative PCRen_US
dc.subject.otherReverse‐Transcription QPCRen_US
dc.subject.otherPerchlorateen_US
dc.titleQuantification of genes and gene transcripts for microbial perchlorate reduction in fixed‐bed bioreactorsen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelMicrobiology and Immunologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationum Department of Civil and Environmental Engineering, University of Michigan, Ann Arbor, MI, USAen_US
dc.contributor.affiliationother Department of Civil, Architectural, and Environmental Engineering, The University of Texas at Austin, Austin, TX, USAen_US
dc.contributor.affiliationother Carollo Engineers, Sarasota, FL, USAen_US
dc.identifier.pmid22188394en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/90307/1/JAM_5225_sm_FigS1.pdf
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/90307/2/JAM_5225_sm_FigS2.pdf
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/90307/3/j.1365-2672.2011.05225.x.pdf
dc.identifier.doi10.1111/j.1365-2672.2011.05225.xen_US
dc.identifier.sourceJournal of Applied Microbiologyen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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