The Importance of Stereochemically Active Lone Pairs For Influencing Pb II and As III Protein Binding
dc.contributor.author | Zampella, Giuseppe | en_US |
dc.contributor.author | Neupane, Kosh P. | en_US |
dc.contributor.author | De gioia, Luca | en_US |
dc.contributor.author | Pecoraro, Vincent L. | en_US |
dc.date.accessioned | 2012-03-16T16:02:00Z | |
dc.date.available | 2013-04-01T14:17:26Z | en_US |
dc.date.issued | 2012-02-13 | en_US |
dc.identifier.citation | Zampella, Giuseppe; Neupane, Kosh P.; De gioia, Luca ; Pecoraro, Vincent L. (2012). "The Importance of Stereochemically Active Lone Pairs For Influencing Pb II and As III Protein Binding." Chemistry â A European Journal 18(7): 2040-2050. <http://hdl.handle.net/2027.42/90414> | en_US |
dc.identifier.issn | 0947-6539 | en_US |
dc.identifier.issn | 1521-3765 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/90414 | |
dc.description.abstract | The toxicity of heavy metals, which is associated with the high affinity of the metals for thiolate rich proteins, constitutes a problem worldwide. However, despite this tremendous toxicity concern, the binding mode of As III and Pb II to proteins is poorly understood. To clarify the requirements for toxic metal binding to metalloregulatory sensor proteins such as As III in ArsR/ArsD and Pb II in PbrR or replacing Zn II in δ‐aminolevulinc acid dehydratase (ALAD), we have employed computational and experimental methods examining the binding of these heavy metals to designed peptide models. The computational results show that the mode of coordination of As III and Pb II is greatly influenced by the steric bulk within the second coordination environment of the metal. The proposed basis of this selectivity is the large size of the ion and, most important, the influence of the stereochemically active lone pair in hemidirected complexes of the metal ion as being crucial. The experimental data show that switching a bulky leucine layer above the metal binding site by a smaller alanine residue enhances the Pb II binding affinity by a factor of five, thus supporting experimentally the hypothesis of lone pair steric hindrance. These complementary approaches demonstrate the potential importance of a stereochemically active lone pair as a metal recognition mode in proteins and, specifically, how the second coordination sphere environment affects the affinity and selectivity of protein targets by certain toxic ions. Experimental and computational methods have been employed to study the influence of the lone pair of As III and Pb II for the binding of these ions in proteins using designed peptide models. The results show that the mode of coordination of As III and Pb II is greatly influenced by the steric bulk within the second coordination environment of the metals (see figure). | en_US |
dc.publisher | WILEY‐VCH Verlag | en_US |
dc.subject.other | Heavy Metal Toxicity | en_US |
dc.subject.other | Lone Pairs | en_US |
dc.subject.other | Selective Binding | en_US |
dc.subject.other | Arsenic | en_US |
dc.subject.other | Lead | en_US |
dc.title | The Importance of Stereochemically Active Lone Pairs For Influencing Pb II and As III Protein Binding | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, Ann Arbor, MI 48109 (USA), Tel.: (+1) 734‐763‐1519, Fax: (+1) 734‐936‐7628 address: | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, Ann Arbor, MI 48109 (USA), Tel.: (+1) 734‐763‐1519, Fax: (+1) 734‐936‐7628 address: | en_US |
dc.contributor.affiliationother | Department of Biotechnology and Biosciences, Università degli Studi di Milano‐Bicocca, Piazza della Scienza 2, 20126, Milano (Italy), Tel: (+39) 02‐64483416, Fax: (+39) 02‐64483478 address: | en_US |
dc.contributor.affiliationother | Department of Biotechnology and Biosciences, Università degli Studi di Milano‐Bicocca, Piazza della Scienza 2, 20126, Milano (Italy), Tel: (+39) 02‐64483416, Fax: (+39) 02‐64483478 address: | en_US |
dc.identifier.pmid | 22231489 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/90414/1/chem_201102786_sm_miscellaneous_information.pdf | |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/90414/2/2040_ftp.pdf | |
dc.identifier.doi | 10.1002/chem.201102786 | en_US |
dc.identifier.source | Chemistry – A European Journal | en_US |
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