Transwells with Microstamped Membranes Produce Micropatterned Two-Dimensional and Three-Dimensional Co-Cultures
dc.contributor.author | Torisawa, Yu-suke | en_US |
dc.contributor.author | Mosadegh, Bobak | en_US |
dc.contributor.author | Cavnar, Stephen P. | en_US |
dc.contributor.author | Ho, Mitchell | en_US |
dc.contributor.author | Takayama, Shuichi | en_US |
dc.date.accessioned | 2012-03-22T17:24:17Z | |
dc.date.available | 2012-03-22T17:24:17Z | |
dc.date.issued | 2011-01-01 | en_US |
dc.identifier.citation | Torisawa, Yu-suke; Mosadegh, Bobak; Cavnar, Stephen P.; Ho, Mitchell; Takayama, Shuichi (2011). "Transwells with Microstamped Membranes Produce Micropatterned Two-Dimensional and Three-Dimensional Co-Cultures." Tissue Engineering Part C: Methods, 17(1): 61-67. <http://hdl.handle.net/2027.42/90499> | en_US |
dc.identifier.issn | 1937-3384 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/90499 | |
dc.description.abstract | This article describes a simple and rapid cell patterning method to form co-culture microarrays in commercially available Transwells. A thin poly(dimethylsiloxane) (PDMS) layer is printed on the underside of a Transwell using a PDMS stamp. Arbitrary cellular patterns are generated according to the geometric features of the thin PDMS layer through hydrodynamic forces that guide cells onto the membrane only over the PDMS-uncoated regions. Micropatterns of surface-adhered cells (we refer to this as two-dimensional) or non-surface-adhered clusters of cells (we refer to this as three-dimensional) can be generated depending on the surface treatment of the filter membrane. Additionally, co-cultures can be established by introducing different types of cells on the membrane or in the bottom chamber of the Transwell. We show that this co-culture method can evaluate mouse embryonic stem (mES) cell differentiation based on heterogeneous cell-cell interactions. Co-culture of mES cells and HepG2 cells decreased SOX17 expression of mES cells, and direct cell-cell contact further decreased SOX17 expression, indicating that co-culture with HepG2 cells inhibits endoderm differentiation through soluble factors and cell-cell contact. This method is simple and user-friendly and should be broadly useful to study cell shapes and cell-cell interactions. | en_US |
dc.publisher | Mary Ann Liebert, Inc., publishers | en_US |
dc.title | Transwells with Microstamped Membranes Produce Micropatterned Two-Dimensional and Three-Dimensional Co-Cultures | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Medicine (General) | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/90499/1/ten-2Etec-2E2010-2E0305.pdf | |
dc.identifier.doi | 10.1089/ten.tec.2010.0305 | en_US |
dc.identifier.source | Tissue Engineering Part C: Methods | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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