Characterization of the flavin–protein interaction in L ‐Lactate oxidase and old yellow enzyme by resonance inverse Raman spectroscopy
dc.contributor.author | Bienstock, Rachelle J. | en_US |
dc.contributor.author | Schopfer, Lawrence M. | en_US |
dc.contributor.author | Morris, Michael D. | en_US |
dc.date.accessioned | 2012-05-21T15:47:26Z | |
dc.date.available | 2012-05-21T15:47:26Z | |
dc.date.issued | 1987-06 | en_US |
dc.identifier.citation | Bienstock, Rachelle J.; Schopfer, Lawrence M.; Morris, Michael D. (1987). "Characterization of the flavin–protein interaction in L ‐Lactate oxidase and old yellow enzyme by resonance inverse Raman spectroscopy." Journal of Raman Spectroscopy 18(4): 241-245. <http://hdl.handle.net/2027.42/91123> | en_US |
dc.identifier.issn | 0377-0486 | en_US |
dc.identifier.issn | 1097-4555 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/91123 | |
dc.description.abstract | Resonance inverse Raman spectroscopy was used to examine the interactions between the flavin and surounding protein in L ‐lactate oxidase (from Mycobacterium smegmatis ) and Old Yellow Enzyme (from brewer's bottom yeast). Spectra were taken of the enzymes both free and bound to various ligands. For L ‐lactate oxidase, the ligands consisted of substrate analogs (acetate, propionate) and inorganic anions (phosphate, sulfate and nitrate). For the inorganic anions, the expected attenuation with detuning was not observed for several bands which are associated with flavin rings II and III. This effect appears to be due to a disruption of ring stacking between the uracil–pyrazine end of the flavin moiety and an aromatic amino acid. A shift in the 1232 cm −1 band and changes in the bandwidths of bands in the 1450–1600 cm −1 region indicate minor reorganization of the hydrogen bonding structure around the isoalloxazine on ligand binding. For Old Yellow Enzyme, the ligand was chloride. Chloride caused a slight change to the Raman spectrum, indicative of decreased hydrogen bonding to the flavin. | en_US |
dc.publisher | John Wiley & Sons, Ltd. | en_US |
dc.title | Characterization of the flavin–protein interaction in L ‐Lactate oxidase and old yellow enzyme by resonance inverse Raman spectroscopy | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Physics | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Departments of Chemistry and Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Departments of Chemistry and Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationother | Texas Instruments Central Research Laboratories, Material Science Laboratory, P.O. Box 225936, Dallas, Texas 75265, USA | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/91123/1/1250180403_ftp.pdf | |
dc.identifier.doi | 10.1002/jrs.1250180403 | en_US |
dc.identifier.source | Journal of Raman Spectroscopy | en_US |
dc.identifier.citedreference | V. Massey, L. M. Schopfer and W. R. Dunham,in Flavins and Flavoproteins,edited by R. C. Bray, P. C. Engel and S. G. Mayhew,pp. 191 – 210. Walter de Gruyter, Berlin ( 1984 ). | en_US |
dc.identifier.citedreference | S. Ghisla and V. Massey, J. Biol. Chem., 250, 577 ( 1975 ). | en_US |
dc.identifier.citedreference | Å. Åkeson, A. Ehrenberg and H. Theorell, The Enzymes, 2nd ed. Vol. 7,pp. 477 – 494. Academic Press, New York ( 1963 ). | en_US |
dc.identifier.citedreference | V. Massey and L. M. Schopfer, J. Biol. Chem., 261, 1215 ( 1986 ). | en_US |
dc.identifier.citedreference | W. J. Rutter and B. Rolander, Acta Chem. Scand., 11, 1663 ( 1957 ). | en_US |
dc.identifier.citedreference | T. Kitagawa, Y. Nishina, K. Shiga, H. Watari and T. Yamano, J. Am. Chem. Soc., 101, 3376 ( 1979 ). | en_US |
dc.identifier.citedreference | Y. Nishina, T. Kitagawa, K. Shiga, H. Watari and T. Yamano, J. Biochem. (Toyko), 87, 831 ( 1980 ). | en_US |
dc.identifier.citedreference | A. S. Abramovitz and V. Massey, J. Biol. Chem., 251, 5321 ( 1976 ). | en_US |
dc.identifier.citedreference | J. K. Eweg, F. Müller and W. J. H. van Berkel, Eur. J. Biochem., 129, 303 ( 1982 ). | en_US |
dc.identifier.citedreference | J. P. Haushalter, G. P. Ritz, D. J. Wallan and M. D. Morris, Appl. Spectrosc., 34, 144 ( 1980 ). | en_US |
dc.identifier.citedreference | C. Buffett and M. D. Morris, Appl. Spectrosc., 35, 203 ( 1981 ). | en_US |
dc.identifier.citedreference | J. P. Haushalter and M. D. Morris, Anal. Chem., 53, 21 ( 1981 ). | en_US |
dc.identifier.citedreference | R. J. Bienstock and M. D. Morris,in Nonlinear Raman Spectroscopy and Its Chemical Applications,edited by W. Kiefer,and D. A. Long,pp. 543 – 559 Reidel, Dordrecht ( 1982 ). | en_US |
dc.identifier.citedreference | R. J. Bienstock, L. M. Schopfer and M. D. Morris, J. Am. Chem. Soc., 108, 1833 ( 1986 ). | en_US |
dc.identifier.citedreference | W. L. Butler, Methods in Enzymol., 24, 3 ( 1972 ). | en_US |
dc.identifier.citedreference | P. A. Sullivan, Y. S. Choong, W. J. Schreurs, J. F. Cutfield, and M. G. Shepherd, Biochem. J., 165, 375 ( 1977 ). | en_US |
dc.identifier.citedreference | P. K. Dutta, J. Nestor and T. G. Spiro, Biochem. Biophys. Res. Commun., 83, 209 ( 1978 ). | en_US |
dc.identifier.citedreference | D. N. Batchelder and D. Bloor,in Advances in Infrared and Raman Spectroscopy,edited by R. E. Hester, and R. J. H. Clark,Vol. 11,pp. 139. Wiley, New York ( 1984 ). | en_US |
dc.identifier.citedreference | Y. Nishimura, M. Tsuboi, Science, 210, 1358 ( 1980 ). | en_US |
dc.identifier.citedreference | K. D. Watenpaugh, L. C. Sukir, and L. H. Jensen, Proc. Natl. Acad. Sci. U.S.A., 70, 3857 ( 1973 ). | en_US |
dc.identifier.citedreference | G. Williamson, P. C. Engel, Y. Nishina and K. Shiga, FEBS Lett., 138, 29 ( 1982 ). | en_US |
dc.identifier.citedreference | R. C. Stewart and V. Massey, J. Biol. Chem., 260, 13639 ( 1985 ). | en_US |
dc.identifier.citedreference | P. K. Dutta, J. Nestor and T. G. Spiro, Proc. Natl. Acad. Sci. U.S.A., 74, 4146 ( 1977 ). | en_US |
dc.identifier.citedreference | P. K. Dutta, R. Spencer, C. Walsh and T. G. Spiro, Biochim. Biophys. Acta, 623, 77 ( 1980 ). | en_US |
dc.identifier.citedreference | L. M. Schopfer, J. P. Haushalter, M. Smith, M. Milad and M. D. Morris, Biochemistry, 20, 6734 ( 1981 ). | en_US |
dc.identifier.citedreference | A. J. W. G. Visser, J. Vervoort, D. O'Kane, J. Lee and L. A. Carriera, Eur. J. Biochem., 31, 639 ( 1983 ). | en_US |
dc.identifier.citedreference | F. Müller, J. Vervoort, M. Horovitz, J. Lee and L. A. Carreira, J. Raman Spectrosc., 14, 106 ( 1983 ). | en_US |
dc.identifier.citedreference | M. Benecky, T. Y. Li, J. Schmidt, F. Frerman, K. L. Watters and J. McFarland, Biochemistry, 18, 3471 ( 1979 ). | en_US |
dc.identifier.citedreference | J. Schmidt, P. Coudron, A. W. Thompson, K. L. Watters and J. McFarland, Biochemistry, 22, 76 ( 1983 ). | en_US |
dc.identifier.citedreference | Y. Nishina, T. Kitagawa, K. Shiga, K. Horlike, Y. Matsumura, H. Watari and T. Yamano, J. Biochem. (Tokyo), 84, 925 ( 1978 ). | en_US |
dc.identifier.citedreference | T. Kitagawa, Y. Nishina, Y. Kyogoku, T. Yamano, N. Ohishi, A. Takai‐Suzuki and K. Yagi, Biochemistry, 18, 1804 ( 1979 ). | en_US |
dc.identifier.citedreference | M. Abe and Y. Kyogoky,in Symposium on Raman Spectroscopy and Biological Science,edited by T. Kitagawa and Y. Kyogoky,p. 42,abstract D3. Osaka University, Osaka, Japan ( 1984 ). | en_US |
dc.identifier.citedreference | W. D. Bowman and T. G. Spiro, Biochemistry, 20, 3313 ( 1981 ). | en_US |
dc.identifier.citedreference | O. Lockridge, V. Massey and P. A. Sullivan, J. Biol. Chem., 247, 8097 ( 1972 ). | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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