Two‐Photon Fluorescence Imaging Super‐Enhanced by Multishell Nanophotonic Particles, with Application to Subcellular pH
dc.contributor.author | Ray, Aniruddha | en_US |
dc.contributor.author | Lee, Yong‐eun Koo | en_US |
dc.contributor.author | Kim, Gwangseong | en_US |
dc.contributor.author | Kopelman, Raoul | en_US |
dc.date.accessioned | 2012-08-09T14:56:44Z | |
dc.date.available | 2013-09-03T15:38:27Z | en_US |
dc.date.issued | 2012-07-23 | en_US |
dc.identifier.citation | Ray, Aniruddha; Lee, Yong‐eun Koo ; Kim, Gwangseong; Kopelman, Raoul (2012). "Twoâ Photon Fluorescence Imaging Superâ Enhanced by Multishell Nanophotonic Particles, with Application to Subcellular pH." Small 8(14): 2213-2221. <http://hdl.handle.net/2027.42/92437> | en_US |
dc.identifier.issn | 1613-6810 | en_US |
dc.identifier.issn | 1613-6829 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/92437 | |
dc.description.abstract | A novel nanophotonic method for enhancing the two‐photon fluorescence signal of a fluorophore is presented. It utilizes the second harmonic (SH) of the exciting light generated by noble metal nanospheres in whose near‐field the dye molecules are placed, to further enhance the dye's fluorescence signal in addition to the usual metal‐enhanced fluorescence phenomenon. This method enables demonstration, for the first time, of two‐photon fluorescence enhancement inside a biological system, namely live cells. A multishell hydrogel nanoparticle containing a silver core, a protective citrate capping, which serves also as an excitation quenching inhibitor spacer, a pH indicator dye shell, and a polyacrylamide cladding are employed. Utilizing this technique, an enhancement of up to 20 times in the two‐photon fluorescence of the indicator dye is observed. Although a significant portion of the enhanced fluorescence signal is due to one‐photon processes accompanying the SH generation of the exciting light, this method preserves all the advantages of infrared‐excited, two‐photon microscopy: enhanced penetration depth, localized excitation, low photobleaching, low autofluorescence, and low cellular damage. The two‐photon fluorescence signal of a fluorophore is enhanced by utilizing the second harmonic of the exciting light generated by noble metal nanospheres in whose near‐field dye molecules are placed. A multishell hydrogel nanoparticle containing a silver core, protective citrate capping, pH indicator dye, and polyacrylamide cladding is utilized for pH sensing and fluorescence imaging in live cells. | en_US |
dc.publisher | WILEY‐VCH Verlag | en_US |
dc.subject.other | Fluorescence | en_US |
dc.subject.other | Photonics | en_US |
dc.subject.other | Second‐Harmonic Generation | en_US |
dc.subject.other | Silver Nanospheres | en_US |
dc.subject.other | Intracellular PH | en_US |
dc.title | Two‐Photon Fluorescence Imaging Super‐Enhanced by Multishell Nanophotonic Particles, with Application to Subcellular pH | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Physics | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | BioPhysics, University of Michigan, 930 N. University Ave. Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, 930 N. University Ave. Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationum | BioPhysics, University of Michigan, 930 N. University Ave. Ann Arbor, MI 48109, USA. | en_US |
dc.identifier.pmid | 22517569 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/92437/1/2213_ftp.pdf | |
dc.identifier.doi | 10.1002/smll.201102664 | en_US |
dc.identifier.source | Small | en_US |
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dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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