A Generic Strategy for Co‐Presentation of Heparin‐Binding Growth Factors Based on CVD Polymerization

Abstract

A multifunctional copolymer with both aldehyde and alkyne groups is synthesized by chemical vapor deposition (CVD) for orthogonal co‐immobilization of biomolecules. Surface analytical methods including FTIR and XPS are used to confirm the surface modification. Heparin‐binding growth factors [basic fibroblast growth factor (bFGF) in this study] can be immobilized through interaction with heparin, which was covalently attached to the CVD surface through an aldehyde‐hydrazide reaction. In parallel, an alkyne–azide reaction is used to orthogonally co‐immobilize an adhesion peptide as the second biomolecule. A generic strategy for immobilizing heparin‐binding growth factors on multifunctional chemical vapor deposition copolymer has been developed. Heparin is covalently attached to the aldehyde‐functionalized surface through a carbohydrazide linker. The growth factor then binds directly to the heparin. The alkyne group can then be used to orthogonally immobilize other biomolecule, such as azido‐functionalized adhesion peptides.