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Experimental reconsideration of the utility of serum starvation as a method for synchronizing mammalian cells

dc.contributor.authorCooper, Stephenen_US
dc.contributor.authorGonzalez‐hernandez, Mariamen_US
dc.date.accessioned2013-02-12T19:00:17Z
dc.date.available2013-02-12T19:00:17Z
dc.date.issued2009-01en_US
dc.identifier.citationCooper, Stephen; Gonzalez‐hernandez, Mariam (2009). "Experimental reconsideration of the utility of serum starvation as a method for synchronizing mammalian cells." Cell Biology International 33(1). <http://hdl.handle.net/2027.42/96238>en_US
dc.identifier.issn1065-6995en_US
dc.identifier.issn1095-8355en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/96238
dc.description.abstractAccurate cell‐size determinations support the prediction that serum starvation and related whole‐culture methods cannot synchronize cells. Theoretical considerations predict that whole‐culture methods of synchronization cannot synchronize cells. Upon serum starvation, the fraction of cells with a G1‐phase amount of DNA increased, but the cell‐size distribution is not narrowed. In true synchronization, the cell‐size distribution should be narrower than the cell‐size distribution of the original culture. In contrast, cells produced by a selective (i.e. non‐whole‐culture) method have a specific DNA content, a narrow size distribution, and divide synchronously. The general theory leading to the conclusion that whole‐culture methods for synchronization do not work implies that one can generalize these serum‐starvation results to other cell lines and other whole‐culture methods, to conclude that these methods do not synchronize cells.en_US
dc.publisherBlackwell Publishing Ltden_US
dc.publisherWiley Periodicals, Inc.en_US
dc.subject.otherG1 Phaseen_US
dc.subject.otherSerum Starvationen_US
dc.subject.otherMembrane Elutionen_US
dc.subject.otherSynchronizationen_US
dc.subject.otherCell Cycleen_US
dc.titleExperimental reconsideration of the utility of serum starvation as a method for synchronizing mammalian cellsen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109‐0620, USAen_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/96238/1/j.cellbi.2008.09.009.pdf
dc.identifier.doi10.1016/j.cellbi.2008.09.009en_US
dc.identifier.sourceCell Biology Internationalen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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