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A reducing redox environment promotes C2C12 myogenesis: Implications for regeneration in aged muscle
dc.contributor.authorHansen, Jason M.en_US
dc.contributor.authorKlass, Markusen_US
dc.contributor.authorHarris, Craigen_US
dc.contributor.authorCsete, Marieen_US
dc.date.accessioned2013-02-12T19:00:22Z
dc.date.available2013-02-12T19:00:22Z
dc.date.issued2007-06en_US
dc.identifier.citationHansen, Jason M.; Klass, Markus; Harris, Craig; Csete, Marie (2007). "A reducing redox environment promotes C2C12 myogenesis: Implications for regeneration in aged muscle." Cell Biology International 31(6). <http://hdl.handle.net/2027.42/96248>en_US
dc.identifier.issn1065-6995en_US
dc.identifier.issn1095-8355en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/96248
dc.description.abstractIntracellular redox potential of skeletal muscle becomes progressively more oxidized with aging, negatively impacting regenerative ability. We examined the effects of oxidizing redox potential on terminal differentiation of cultured C2C12 myoblasts. Redox potentials were manipulated by changing the culture O 2 environment, by free radical scavenging, or addition of H 2 O 2. Intracellular reactive oxygen species (ROS) production was higher in 20% environmental O 2 and in this condition, redox potential became progressively oxidized compared to cultures in 6% O 2. Treatment with a ROS trapping agent (phenyl‐ N‐tert ‐butylnitrone, PBN) caused reducing redox potentials and enhanced C2C12 differentiation, while addition of 25 micromolar H 2 O 2 to cells in 20% O 2 dramatically slowed differentiation. Under these most oxidative conditions, quantitative PCR showed a significant decrease in myogenic basic helix—loop—helix transcription factor expression compared to cultures treated with PBN or grown in 6% O 2 . Thus, oxidative intracellular environments impair myoblast differentiation, while reducing environments favor myogenesis.en_US
dc.publisherBlackwell Publishing Ltden_US
dc.publisherWiley Periodicals, Inc.en_US
dc.subject.otherRedox Potentialen_US
dc.subject.otherMyogeninen_US
dc.subject.otherMyoblasten_US
dc.subject.otherMuscle Agingen_US
dc.subject.otherC2C12 Myoblasten_US
dc.titleA reducing redox environment promotes C2C12 myogenesis: Implications for regeneration in aged muscleen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumToxicology Program, Department of Environmental Health Sciences, School of Public Health, University of Michigan, Ann Arbor, MI 48109, USAen_US
dc.contributor.affiliationotherDepartment of Anesthesiology, School of Medicine, Emory University, 1462 Clifton Road NE, Suite 420, Atlanta, GA 30322, USAen_US
dc.identifier.pmid17241791en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/96248/1/j.cellbi.2006.11.027.pdf
dc.identifier.doi10.1016/j.cellbi.2006.11.027en_US
dc.identifier.sourceCell Biology Internationalen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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