In Vitro Differentiation and Mineralization of Dental Pulp Stem Cells on Enamel-Like Fluorapatite Surfaces
dc.contributor.author | Wang, Xiaodong | en_US |
dc.contributor.author | Jin, Taocong | en_US |
dc.contributor.author | Chang, Syweren | en_US |
dc.contributor.author | Zhang, Zhaocheng | en_US |
dc.contributor.author | Czajka-Jakubowska, Agata | en_US |
dc.contributor.author | Nör, Jacques E. | en_US |
dc.contributor.author | Clarkson, Brian H. | en_US |
dc.contributor.author | Ni, Longxing | en_US |
dc.contributor.author | Liu, Jun | en_US |
dc.date.accessioned | 2013-06-25T18:43:23Z | |
dc.date.available | 2013-06-25T18:43:23Z | |
dc.date.issued | 2012-11 | en_US |
dc.identifier.citation | Wang, Xiaodong; Jin, Taocong; Chang, Syweren; Zhang, Zhaocheng; Czajka-Jakubowska, Agata; Nör, Jacques E.; Clarkson, Brian H.; Ni, Longxing; Liu, Jun (2012). "In Vitro Differentiation and Mineralization of Dental Pulp Stem Cells on Enamel-Like Fluorapatite Surfaces." Tissue Engineering Part C: Methods 18(11): 821-830. <http://hdl.handle.net/2027.42/98483> | en_US |
dc.identifier.issn | 1937-3384 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/98483 | |
dc.description.abstract | Our previous studies have shown good biocompatibility of fluorapatite (FA) crystal surfaces in providing a favorable environment for functional cell?matrix interactions of human dental pulp stem cells (DPSCs) and also in supporting their long-term growth. The aim of the current study was to further investigate whether this enamel-like surface can support the differentiation and mineralization of DPSCs, and, therefore, act as a potential model for studying the enamel/dentin interface and, perhaps, dentine/pulp regeneration in tooth tissue engineering. The human pathway-focused osteogenesis polymerase chain reaction (PCR) array demonstrated that the expression of osteogenesis-related genes of human DPSCs was increased on FA surfaces compared with that on etched stainless steel (SSE). Consistent with the PCR array, FA promoted mineralization compared with the SSE surface with or without the addition of a mineralization promoting supplement (MS). This was confirmed by alkaline phosphatase (ALP) staining, Alizarin red staining, and tetracycline staining for mineral formation. In conclusion, FA crystal surfaces, especially ordered (OR) FA surfaces, which mimicked the physical architecture of enamel, provided a favorable extracellular matrix microenvironment for the cells. This resulted in the differentiation of human DPSCs and mineralized tissue formation, and, thus, demonstrated that it may be a promising biomimetic model for dentin-pulp tissue engineering. | en_US |
dc.publisher | Mary Ann Liebert, Inc., publishers | en_US |
dc.title | In Vitro Differentiation and Mineralization of Dental Pulp Stem Cells on Enamel-Like Fluorapatite Surfaces | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Medicine (General) | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.identifier.pmid | 22563788 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/98483/1/ten%2Etec%2E2011%2E0624.pdf | |
dc.identifier.doi | 10.1089/ten.tec.2011.0624 | en_US |
dc.identifier.source | Tissue Engineering Part C: Methods | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.