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Orosphere assay: A method for propagation of head and neck cancer stem cells

dc.contributor.authorKrishnamurthy, Sudhaen_US
dc.contributor.authorNör, Jacques E.en_US
dc.date.accessioned2013-07-08T17:45:30Z
dc.date.available2014-09-02T14:12:52Zen_US
dc.date.issued2013-07en_US
dc.identifier.citationKrishnamurthy, Sudha; Nör, Jacques E. (2013). "Orosphere assay: A method for propagation of head and neck cancer stem cells." Head & Neck 35(7): 1015-1021. <http://hdl.handle.net/2027.42/98785>en_US
dc.identifier.issn1043-3074en_US
dc.identifier.issn1097-0347en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/98785
dc.description.abstractBackground Recent evidence suggests that head and neck squamous cell carcinomas (HNSCCs) harbor a small subpopulation of highly tumorigenic cells, designated cancer stem cells. A limiting factor in cancer stem cell research is the intrinsic difficulty of expanding cells in an undifferentiated state in vitro. Methods Here, we describe the development of the orosphere assay, a method for the study of putative head and neck cancer stem cells. An orosphere is defined as a nonadherent colony of cells sorted from primary HNSCCs or from HNSCC cell lines and cultured in 3‐dimensional soft agar or ultralow attachment plates. Aldehyde dehydrogenase activity and CD44 expression were used here as stem cell markers. Results This assay allowed for the propagation of head and neck cancer cells that retained stemness and self‐renewal. Conclusion The orosphere assay is well suited for studies designed to understand the pathobiology of head and neck cancer stem cells. © 2012 Wiley Periodicals, Inc. Head Neck, 2013en_US
dc.publisherWiley Subscription Services, Inc., A Wiley Companyen_US
dc.subject.otherSuspension Cultureen_US
dc.subject.otherStemnessen_US
dc.subject.otherSelf‐Renewalen_US
dc.subject.otherSphereen_US
dc.subject.otherSquamous Cell Carcinomaen_US
dc.titleOrosphere assay: A method for propagation of head and neck cancer stem cellsen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelOtolaryngologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumProfessor of Dentistry, Otolaryngology, and Biomedical Engineering, University of Michigan, Ann Arbor, Michiganen_US
dc.contributor.affiliationumDepartment of Biomedical Engineering, University of Michigan College of Engineering, Ann Arbor, Michiganen_US
dc.contributor.affiliationumDepartment of Otolaryngology, University of Michigan School of Medicine, Ann Arbor, Michiganen_US
dc.contributor.affiliationumDepartment of Restorative Sciences, University of Michigan School of Dentistry, Ann Arbor, Michiganen_US
dc.identifier.pmid22791367en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/98785/1/23076_ftp.pdf
dc.identifier.doi10.1002/hed.23076en_US
dc.identifier.sourceHead & Necken_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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