Purification of bovine liver microsomal NADH-cytochrome  reductase using affinity chromatography

Purification of bovine liver microsomal NADH-cytochrome reductase using affinity chromatography

Schafer, Dorothy A.; Hultquist, Donald E.

1980-07-16

Citation: Schafer, Dorothy A., Hultquist, Donald E. (1980/07/16)."Purification of bovine liver microsomal NADH-cytochrome reductase using affinity chromatography." Biochemical and Biophysical Research Communications 95(1): 381-387. <http://hdl.handle.net/2027.42/23200>

Abstract: Microsomal NADH-cytochrome reductase has been purified from bovine liver by an improved procedure which employs affinity chromatography on ADP-agarose in combination with anion exchange chromatography. The reductase was extracted from a 105,000 x microsomal pellet with Triton X-100. The overall purification from isolated microsomes was 98-fold and the yield was 10%. The preparation was nearly homogeneous on SDS-PAGE. This procedure requires less time and effort than previously described procedures. Partially purified cytochrome is also obtained.

DOIs: http://dx.doi.org/10.1016/0006-291X(80)90749-4

PMID: 7417264

URI: http://www.sciencedirect.com/science/article/B6WBK-4DN95M1-10S/2/a9e05830baa2ffa5e7ab35ce4b1a806c
http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7417264&dopt=citation

Handle: http://hdl.handle.net/2027.42/23200

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