Protein extraction using the sodium bis(2-ethylhexyl) phosphate (NaDEHP) reverse micellar system
dc.contributor.author | Hu, Ziyi | en_US |
dc.contributor.author | Gulari, Erdogan | en_US |
dc.date.accessioned | 2006-04-28T16:31:58Z | |
dc.date.available | 2006-04-28T16:31:58Z | |
dc.date.issued | 1996-04-20 | en_US |
dc.identifier.citation | Hu, Ziyi; Gulari, Erdogan (1996)."Protein extraction using the sodium bis(2-ethylhexyl) phosphate (NaDEHP) reverse micellar system." Biotechnology and Bioengineering 50(2): 203-206. <http://hdl.handle.net/2027.42/37936> | en_US |
dc.identifier.issn | 0006-3592 | en_US |
dc.identifier.issn | 1097-0290 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/37936 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=18626936&dopt=citation | en_US |
dc.description.abstract | The reverse micellar system of sodium bis(2-ethylhexyl) phosphate (NaDEHP)/isooctane/brine was used for liquid-liquid extraction of proteins. We investigated the solubilization of cytochrome-c and Α-chymotrypsin into the NaDEHP reverse micellar phase by varying the pH and NaCl concentration in the aqueous phase. At neutral pH and relatively low ionic strength, the proteins are extracted into the micellar phase with high yield. By contacting the micellar phase with a divalent cation (e.g., Ca 2+ ) aqueous solution, the reverse micelles are destabilized and release the protein molecules back into an aqueous solution for recovery. This method separates the proteins from the surfactant with very high overall efficiencies. © 1996 John Wiley & Sons, Inc. | en_US |
dc.format.extent | 414577 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Biochemistry and Biotechnology | en_US |
dc.title | Protein extraction using the sodium bis(2-ethylhexyl) phosphate (NaDEHP) reverse micellar system | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbsecondlevel | Mathematics | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Statistics and Numeric Data | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Social Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Chemical Engineering, University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Department of Chemical Engineering, University of Michigan, Ann Arbor, Michigan 48109 ; Department of Chemical Engineering, University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.identifier.pmid | 18626936 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/37936/1/9_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/(SICI)1097-0290(19960420)50:2<203::AID-BIT9>3.0.CO;2-Q | en_US |
dc.identifier.source | Biotechnology and Bioengineering | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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