A rapid method for detection of Y-chromosomal DNA from dried blood specimens by the polymerase chain reaction
dc.contributor.author | Witt, Michal P. | en_US |
dc.contributor.author | Erickson, Robert P. | en_US |
dc.date.accessioned | 2006-09-11T19:12:19Z | |
dc.date.available | 2006-09-11T19:12:19Z | |
dc.date.issued | 1989-06 | en_US |
dc.identifier.citation | Witt, Michal; Erickson, Robert P.; (1989). "A rapid method for detection of Y-chromosomal DNA from dried blood specimens by the polymerase chain reaction." Human Genetics 82(3): 271-274. <http://hdl.handle.net/2027.42/47625> | en_US |
dc.identifier.issn | 1432-1203 | en_US |
dc.identifier.issn | 0340-6717 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/47625 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2567276&dopt=citation | en_US |
dc.description.abstract | The alphoid satellite family is the only repetitive DNA family showing chromosome specificity. We have developed a simple, rapid, and reliable test for sex diagnosis based on detection of these sequences in undigested genomic DNA using the polymerase chain reaction. In our test, dried blood specimens were the source of DNA. When female DNA was used as a template for the reaction, only the expected 130-bp X-chromosome-specific fragment was detected, while with male DNA both the expected 170-bp Y-chromosome-specific and X-chromosome-specific fragments were detected. The Y-chromosome-specific fragment was further characterized by restriction enzyme analysis. The Y fragment was detectable when DNA obtained from an equivalent of 10 μl of spotted blood was used in the reaction, whereas detection of the X fragment was possible with DNA from an equivalent of 5 μl of blood. Our test may find various applications in newborn screening and in forensic science. | en_US |
dc.format.extent | 599934 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag | en_US |
dc.subject.other | Metabolic Diseases | en_US |
dc.subject.other | Human Genetics | en_US |
dc.subject.other | Biomedicine | en_US |
dc.subject.other | Molecular Medicine | en_US |
dc.subject.other | Internal Medicine | en_US |
dc.title | A rapid method for detection of Y-chromosomal DNA from dried blood specimens by the polymerase chain reaction | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Genetics | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan Medical School, 48109, Ann Arbor, MI, USA; Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, 48109, Ann Arbor, MI, USA; Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan Medical School, 48109, Ann Arbor, MI, USA; Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, 48109, Ann Arbor, MI, USA; Division of Pediatric Genetics, Department of Human Genetics, Medical Science II M4708, 1500 East Medical Center Drive, 48109-0618, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 2567276 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/47625/1/439_2004_Article_BF00291168.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF00291168 | en_US |
dc.identifier.source | Human Genetics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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