Now showing items 1-10 of 48
Rapid protein release from Escherichia coli by chemical permeabilization under fermentation conditions
(Wiley Subscription Services, Inc., A Wiley Company, 1992-03-25)
Overall protein release greater than 75% in less than 1 h can be attained by exposing exponentially growing Escherichia coli cells to 0.4 M guanidine plus 0.5% Triton X-100 at 37°C in medium. Cell growth stops immediately ...
The effects of exopolymers on cell morphology and culturability of Leuconostoc mesenteroides during starvation
(Springer-Verlag; Springer-Verlag Berlin Heidelberg, 1999-11)
Biofilm formation by bacterial cells can be used to modify the subsurface permeability for the purpose of microbial enhanced oil recovery, bio-barrier formation, and in situ bioremediation. Once injected into the subsurface, ...
Quantitative evaluation of the pH profile in organic acid fermentations
(Wiley Subscription Services, Inc., A Wiley Company, 1991-01-20)
No Abstrct.
Recovery of a foreign protein from the periplasm of Escherichia coli by chemical permeabilization
(Elsevier, 1990-08)
We have applied the technique of protein release by chemical permeabilization to recover a foreign protein in active form from the periplasm of a recombinant strain of Escherichia coli. The two agents used in our chemical ...
Extraction of aminoglycoside antibiotics with reverse micelles
(John Wiley & Sons, Ltd., 1996-01)
The reverse micelle system of sodium di-2-ethylhexyl phosphate was used to extract aminoglycoside antibiotics, neomycin and gentamicin. The aminoglycosides can be efficiently extracted into a reverse micelle solution, and ...
Selective extraction using preferential transport through adsorptive membranes
(Wiley Subscription Services, Inc., A Wiley Company, 1996-12-05)
Selective extraction of a protein from a mixture can be accomplished using an adsorptive membrane and low displacement recuperative parametric pumping. Low displacement recuperative parametric pumping can lead to the ...
Protein separation using membrane-encapsulated soluble ligand conjugates
(Elsevier, 1990-05)
A new approach for isolating and recovering biological macromolecules using membrane-encapsulated soluble ligand conjugates was investigated. Membrane-encapsulated solid adsorbents have been successfully developed and ...
Chemical permeabilization and in situ removal of daidzein from biologically viable soybean (Glycine max) seeds
(Kluwer Academic Publishers; Chapman and Hall ; Springer Science+Business Media, 1998-09)
After 24 h of chemical permeabilization with 20% (v/v) methanol at 25 °C, the amount of daidzein released from soybean seeds is 15 to 20% of the amount (0.0423 ± 0.0045 mg/g seed dry wt) obtained by physical grinding. With ...
Fe---P---0 catalysts for methane utilization--catalyst development and identification
(Elsevier, 1990-05)
Fe---P---0 catalyst formulations show great promise for oxidative coupling of methane. They attain yields of C2 hydrocarbons comparable to the best in the literature at a temperature which is far below those reported. The ...
Protein extraction using the sodium bis(2-ethylhexyl) phosphate (NaDEHP) reverse micellar system
(Wiley Subscription Services, Inc., A Wiley Company, 1996-04-20)
The reverse micellar system of sodium bis(2-ethylhexyl) phosphate (NaDEHP)/isooctane/brine was used for liquid-liquid extraction of proteins. We investigated the solubilization of cytochrome-c and Α-chymotrypsin into the ...