Now showing items 11-20 of 63
[lambda] N antitermination system: Functional analysis of phage interactions with the host NusA protein
(Elsevier, 1987-04-20)
Coliphage [lambda] gene expression is regulated temporally by systems of termination and antitermination of transcription. The [lambda]-encoded N protein (pN) acting with host factors (Nus) at sites (nut) located downstream ...
Novel shuttle plasmid vehicles for Escherichia-Streptococcus transgeneric cloning
(Elsevier, 1983-11)
A novel plasmid vector that is able to replicate both in Escherichia coli and in Streptococcus sanguis is described. This 9.2-kb plasmid, designated pVA856, carries Cmr, Tcr and Emr determinants that are expressed in E. ...
Isolation and structure of the Streptococcus faecalis sex pheromone, cAM373
(Elsevier, 1986-09-29)
The Streptococcus faecalis sex pheromone, cAM373, which induces a mating response of donor cells harboring plasmid pAM373 and is also produced by Staphylococcus aureus, was isolated and its structure determined. Supernatant ...
A cloning vector able to replicate in Escherichia coli and Streptococcus sanguis
(Elsevier, 1982-10)
A plasmid that is able to replicate in both Escherichia coli and Streptococcus sanguis has been constructed by the in vitro joining of the pACYC184 (Cmr Tcr) and pVA749 (Emr) replicons. This plasmid, designated pVA838, is ...
Am E. coli gene product required for [lambda] site-specific recombination
(Elsevier, 1980-07)
We report characteristics of himA mutations of E. coli, selected for their inability to support the site-specific recombination reaction involved in the formation of lysogens by bacteriophage [lambda]. The himA allele lies ...
Efficient and quantitative incorporation of diaminopimelic acid into the peptidoglycan of Salmonella typhimurium
(Blackwell Publishing Ltd, 1986-09)
Diaminopimelic acid is incorporated into the peptidoglycan of Salmonella typhimurium in an efficient and quantitative manner. The amount of DAP incorporated is similar to the number of molecules estimated to exist in the ...
Identification of the nus B gene product of Escherichia coli
(Springer-Verlag; Springer-Verlag GmbH & Co. KG, 1981-08)
Escherichia coli nus B mutants fail to support the activity of a phage λ gene product, pN, which regulates phage gene expression by influencing transcription termination. We report the identification of the nus B protein ...
Reconstitution of a cyclic amp-dependet protein kinase from Dictyostellium discoideum
(Elsevier, 1983-07-28)
Dicytostelium discoideum has a cyclic AMP-dependent protein kinase activity which can be assayed after DEAE-ion exchange chromatography. Mixing cAMP binding protein (free of kinase activity) from the DEAE column eluate ...
Identification and characterization of the adenosine 3',5'-cyclic monophosphate binding proteins appearing during the development of Dictyostelium discoideum
(Elsevier, 1980-05-07)
A photosensitive, radioactive analogue of cyclic adenosine monophosphate, 8-azido-adenosine 3',5'-[32P]monophosphate (8-N3-cyclic AMP), was used to label the cyclic AMP binding proteins of Dictyostelium discoideum. During ...
Dominance of iminopeptidase activity in the human oral bacterium Treponema denticola ATCC 35405
(Springer-Verlag, 1986-11)
Treponema denticola ATCC 35405, a human oral spirochete associated with periodontal disease, was shown to contain three enzymes (I, II, and III) with proline iminopeptidase activity. II and III were considered to be true ...