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- Creator:
- Ludlow, Andrew
- Description:
- Single molecule long read RNA/cDNA sequencing of TERT revealed 45 TERT mRNA variants including 13 known and 32 novel variants. Among the variants, TERT Delta 2-4, which lacks exons 2-4 but retains the original open reading frame, was selected for further study. Induced pluripotent stem cells and cancer cells express higher levels of TERT Delta 2-4 compared to primary human bronchial epithelial cells. Overexpression of TERT Delta 2-4 enhanced clonogenicity and resistance to cisplatin-induced apoptosis. Knockdown of endogenous TERT Delta 2-4 in Calu-6 cells reduced clonogenicity and resistance to cisplatin. Our results suggest that TERT Delta 2-4 enhances cancer cells’ resistance to intrinsic apoptosis. RNA sequencing following knockdown of Delta 2-4 TERT indicates that translation is downregulated and that mitochondrial related proteins are upregulated compared to controls.
- Keyword:
- TERT, Alternative splicing, Telomere, and Telomerase
- Discipline:
- Science
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- Creator:
- Chen, Hongfan, Sachdeva, Nishtha, Huang, Zhenguang, van der Holst, Bart, Manchester, Ward, Jivani, Aniket, Zou, Shasha, Chen, Yang, Huan, Xun, and Toth, Gabor
- Description:
- In this study, we show that coronal mass ejection (CME) simulations conducted with the Space Weather Modeling Framework (SWMF) can be assimilated with SOHO LASCO white-light (WL) coronagraph observations and solar wind observations at L1 prior to the CME eruption to improve the prediction of CME arrival time. L1 observations are used to constrain the background solar wind, while LASCO coronagraph observations filter the initial ensemble simulations by constraining the simulated CME propagation speed. We then construct probabilistic predictions for CME arrival time using the data-assimilated ensemble. Scripts in this work are written in R, Python and Julia.
- Keyword:
- Data Assimilation, Uncertainty Quantification, and Space Weather
- Discipline:
- Engineering
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- Creator:
- Hawes, Jason K
- Description:
- Most of this deposit is composed of a step-by-step explanation of how to replicate the work conducted in Chapters 2 and 5 of my dissertation (available in DeepBlue Documents under the title Urban Agriculture: Good for People, Places, and Planet?). Very little actual data is catalogued here, instead largely relying on links to the secondary datasets online. In fact, this is an intentional choice, since any replication would likely want updated data to produce more real-time results. This deposit is intended to accompany the dissertation and may not be the final version of these two manuscripts or their associated methods. For more up-to-date methods and analysis, please search Google Scholar or your affiliated library for Jason Hawes and some combination of keywords including urban agriculture, scaling-up, tradeoffs, or the names of the cities in question.
- Keyword:
- urban agriculture, tradeoffs, remote sensing, and multi-criteria analysis
- Discipline:
- Social Sciences
-
- Creator:
- Crowell, Hayley L . , Curlis, John David, Weller, Hannah I., and Davis Rabosky, Alison R.
- Description:
- Ultraviolet (UV) wavelengths invisible to humans are primarily studied in the context of reproduction and social signaling. This narrow focus can arise from bias in taxa studied, which are often brightly colored in human-visible wavelengths. Our research describes undocumented UV color patterns across 110 diverse species of Western Hemisphere snakes and tests the hypothesized roles of reproduction versus predator avoidance in the evolution of UV coloration. Phylogenetically-informed tests of life stage, sex, and habitat showed unexpected support for the predator defense hypothesis, with pronounced differences in snake conspicuousness explained by UV coloration. UV reflectance was not predictable from any aspect of visible color pattern, suggesting high potential for transformative discoveries in other “cryptically-colored” lineages across the tree of life.
- Keyword:
- color, ecology, evolution, predators, snakes, and ultraviolet
- Citation to related publication:
- Crowell, H. L., Curlis, J. D., Weller, H. I., & Davis Rabosky, A. R. (2024). Ecological drivers of ultraviolet colour evolution in snakes. Nature Communications, 15(1), 5213. http://doi.org/10.1038/s41467-024-49506-4
- Discipline:
- Science
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Estimates of the water balance of the Laurentian Great Lakes using the Large Lakes Statistical Water Balance Model (L2SWBM)
User Collection
- Creator:
- Smith, Joeseph P., Fry, Lauren M., Do, Hong X., and Gronewold, Andrew D.
- Description:
- This collection contains estimates of the water balance of the Laurentian Great Lakes that were produced by the Large Lakes Statistical Water Balance Model (L2SWBM). Each data set has a different configuration and was used as the supplementary for a published peer-reviewed article (see "Citations to related material" section in the metadata of individual data sets). The key variables that were estimated by the L2SWBM are (1) over-lake precipitation, (2) over-lake evaporation, (3) lateral runoff, (4) connecting-channel outflows, (5) diversions, and (6) predictive changes in lake storage. and Contact: Andrew Gronewold Office: 4040 Dana Phone: (734) 764-6286 Email: drewgron@umich.edu
- Keyword:
- Great Lakes water levels, statistical inference, water balance, data assimilation, Great Lakes, Laurentian, Machine learning, Bayesian, and Network
- Citation to related publication:
- Smith, J. P., & Gronewold, A. D. (2017). Development and analysis of a Bayesian water balance model for large lake systems. arXiv preprint arXiv:1710.10161., Gronewold, A. D., Smith, J. P., Read, L., & Crooks, J. L. (2020). Reconciling the water balance of large lake systems. Advances in Water Resources, 103505., and Do, H.X., Smith, J., Fry, L.M., and Gronewold, A.D., Seventy-year long record of monthly water balance estimates for Earth’s largest lake system (under revision)
- Discipline:
- Science and Engineering
5Works -
- Creator:
- Fu, Xun, Zhang, Bohao, Weber, Ceri J., Cooper, Kimberly L., Vasudevan, Ram, and Moore, Talia Y.
- Description:
- Tails used as inertial appendages induce body rotations of animals and robots---a phenomenon that is governed largely by the ratio of the body and tail moments of inertia. However, vertebrate tails have more degrees of freedom (e.g., number of joints, rotational axes) than most current theoretical models and robotic tails. To understand how morphology affects inertial appendage function, we developed an optimization-based approach that finds the maximally effective tail trajectory and measures error from a target trajectory. For tails of equal total length and mass, increasing the number of equal-length joints increased the complexity of maximally effective tail motions. When we optimized the relative lengths of tail bones while keeping the total tail length, mass, and number of joints the same, this optimization-based approach found that the lengths match the pattern found in the tail bones of mammals specialized for inertial maneuvering. In both experiments, adding joints enhanced the performance of the inertial appendage, but with diminishing returns, largely due to the total control effort constraint. This optimization-based simulation can compare the maximum performance of diverse inertial appendages that dynamically vary in moment of inertia in 3D space, predict inertial capabilities from skeletal data, and inform the design of robotic inertial appendages.
- Keyword:
- simulation, inertial maneuvering, caudal vertebrae, trajectory optimization, and reconfigurable appendages
- Citation to related publication:
- Xun Fu, Bohao Zhang, Ceri J. Weber, Kimberly L. Cooper, Ram Vasudevan, Talia Y. Moore. (in review) Jointed tails enhance control of three-dimensional body rotation.
- Discipline:
- Engineering and Science
-
- Creator:
- Sorensen, Troy R, Espey, Eamon, Kelley, John G. W. , Kessler, James, and Gronewold, Andrew D.
- Description:
- Inland lakes play a critical role in ecosystem stability, and robust validation of lake models is essential for understanding their dynamics. While remote sensing data can assist with lake surface temperature validation, in situ data typically provides more accurate, reliable data not limited to only the lake surface. However, in situ temperature data for many individual lakes, particularly in North America, is difficult for researchers to quickly access in a standardized format. This database offers a well-organized collection of in situ near-surface and subsurface temperatures from 134 sites divided among 29 large North American inland lakes collected from a variety of sources. The database includes multiple subsurface temperatures throughout the depth profile of 84 of these sites, providing comprehensive data for lake model evaluation. All lakes selected for this database are large enough (over approximately 30 km^2 to be represented by large-scale operational weather models, supporting robust lake model validation efforts on the lakes that have the greatest impact on climatology.
- Keyword:
- lake, temperature, in situ, and subsurface
- Citation to related publication:
- Sorensen, T., Espey, E., Kelley, J.G.W. et al. A database of in situ water temperatures for large inland lakes across the coterminous United States. Sci Data 11, 282 (2024). https://doi.org/10.1038/s41597-024-03103-8
- Discipline:
- Science
-
- Creator:
- Do, Hong X., Smith, Joeseph P., Fry, Lauren M., and Gronewold, Andrew D.
- Description:
- This data set contains a new monthly estimate of the water balance of the Laurentian Great Lakes, the largest freshwater system on Earth, from 1950 to 2019. The source codes and inputs to derive the new estimates are also included in this dataset. and ***ADDED 2024-02-27: The component net basins supply data "*NBSC_GLWBData.csv" in "output_ts_posterior.zip" need to be revised for further applications***
- Keyword:
- Laurentian Great Lakes, Bayesian inference, water levels, data assimilation, and water balance
- Citation to related publication:
- Do, H. X., Smith, J. P., Fry, L. M., & Gronewold, A. D. (2020). Seventy-year long record of monthly water balance estimates for Earth’s largest lake system. Scientific Data, 7(1), 276. https://doi.org/10.1038/s41597-020-00613-z, Gronewold, A. D., Smith, J. P., Read, L., & Crooks, J. L. (2020). Reconciling the water balance of large lake systems. Advances in Water Resources, 103505. https://doi.org/10.1016/j.advwatres.2020.103505 , and This version replaces the following deprecated dataset: Do, H.X., Smith, J.P., Fry, L.M., Gronewold, A.D. (2020). Monthly water balance estimates for the Laurentian Great Lakes from 1950 to 2019 [Data set]. University of Michigan - Deep Blue. https://doi.org/10.7302/0rsp-v195
- Discipline:
- Science
-
- Creator:
- Srodawa, Kristy, Cerda, Peter A, Davis Rabosky, Alison R, and Crowe-Riddell, Jenna M
- Description:
- Snake venom research has historically focused on front-fanged species (Viperidae and Elapidae), limiting our knowledge of venom evolution in rear-fanged snakes across their ecologically-diverse phylogeny. Three finger toxins (3FTxs) are a known neurotoxic component in the venoms of some rear-fanged snakes (Colubrinae, Colubridae), but it is unclear how prevalent 3FTxs are both in expression within venom glands and more broadly among colubrine species. Here, we used a transcriptomic approach to characterize the venom expression profiles of four species of colubrine snakes from Neotropics that were dominated by 3FTx expression (in the genera Chironius, Oxybelis, Rhinobothryum, and Spilotes) and reconstructed the gene trees of 3FTxs. Overall, our results highlight the importance of exploring the venoms of understudied species in reconstructing the full evolutionary history of toxins across the tree of life.
- Keyword:
- snake venom, neurotoxin, molecular evolution, gene families, and opisthoglyphous
- Citation to related publication:
- Srodawa, K., Cerda, P.A., Davis Rabosky, A.R., Crowe-Riddell, J.M. Evolution of Three Finger Toxin Genes in Neotropical Colubrine Snakes (Colubridae). Toxins 2023, 15(9), 523; https://doi.org/10.3390/toxins15090523
- Discipline:
- Science
-
- Creator:
- Moore, Talia Y, Villacis Nunez, C Nathaly, Ray, Andrew P, and Cooper, Kimberly L
- Description:
- Hind limbs can undergo dramatic changes in loading conditions during the transition from quadrupedal to bipedal locomotion. For example, the most early diverging bipedal jerboas (Rodentia: Dipodidae) are some of the smallest mammals in the world, with body masses that range 2-4 grams. The larger jerboa species exhibit developmental and evolutionary fusion of the central three metatarsals into a single cannon bone. We hypothesize that body size reduction and metatarsal fusion are mechanisms to maintain the safety factor of the hind limb bones despite the higher ground reaction forces associated with bipedal locomotion. Using finite element analysis to model collisions between the substrate and the metatarsals, we found that body size reduction was insufficient to reduce bone stress on unfused metatarsals, based on the scaled dynamics of larger jerboas, and that fused bones developed lower stresses than unfused bones when all metatarsals are scaled to the same size and loading conditions. Based on these results, we conclude that fusion reinforces larger jerboa metatarsals against high ground reaction forces. Because smaller jerboas with unfused metatarsals develop higher peak stresses in response to loading conditions scaled from larger jerboas, we hypothesize that smaller jerboas use alternative dynamics of bipedal locomotion that reduces the impact of collisions between the foot and substrate.
- Keyword:
- finite element, functional morphology, bipedal, jerboa, metatarsus, and bone fusion
- Citation to related publication:
- Villacis Nunez, Ray, Cooper, Moore (submitted). Body size reduction and metatarsal fusion were distinct mechanisms to resist bending as jerboas (Dipodidae) transitioned from quadrupedal to bipedal.
- Discipline:
- Science and Engineering