Scan of specimen ummz:mammals:101779 (Callorhinus ursinus) - WholeBody. Reconstructed Dataset includes 1883 TIF images (each 1927 x 1392 x 1 voxel at 0.121618 mm resolution, derived from 1601 scan projections), xtek and vgi files for volume reconstruction. and Scan of specimen ummz:mammals:101779 (Callorhinus ursinus) - WholeBody. Raw Dataset includes 1601 TIF images (each 1927 x 1392 x 1 voxel at 0.1216177 mm resolution, derived from 1601 scan projections), xtek and vgi files for volume reconstruction.
Scan of specimen ummz:mammals:103553 (Cynictis penicillata) - WholeBody. Raw Dataset includes 1601 TIF images (each 1048 x 1049 x 1 voxel at 0.03995863 mm resolution, derived from 1601 scan projections), xtek and vgi files for volume reconstruction. and Scan of specimen ummz:mammals:103553 (Cynictis penicillata) - WholeBody. Reconstructed Dataset includes 1946 TIF images (each 1048 x 1049 x 1 voxel at 0.039959 mm resolution, derived from 1601 scan projections), xtek and vgi files for volume reconstruction.
Scan of specimen ummz:mammals:114800 (Acinonyx jubatus) - WholeBody. Raw Dataset includes 1601 TIF images (each 1978 x 1688 x 1 voxel at 0.09413704 mm resolution, derived from 1601 scan projections), xtek and vgi files for volume reconstruction. and Scan of specimen ummz:mammals:114800 (Acinonyx jubatus) - WholeBody. Reconstructed Dataset includes 1671 TIF images (each 1978 x 1688 x 1 voxel at 0.094137 mm resolution, derived from 1601 scan projections), xtek and vgi files for volume reconstruction.
Scan of specimen ummz:mammals:157158 (Conepatus humboldtii) - WholeBody. Reconstructed Dataset includes 1979 TIF images (each 984 x 1237 x 1 voxel at 0.037468 mm resolution, derived from 1601 scan projections), xtek and vgi files for volume reconstruction. and Scan of specimen ummz:mammals:157158 (Conepatus humboldtii) - WholeBody. Raw Dataset includes 1601 TIF images (each 984 x 1237 x 1 voxel at 0.03746828 mm resolution, derived from 1601 scan projections), xtek and vgi files for volume reconstruction.
Scan of specimen ummz:mammals:163808 (Crocuta crocuta) - WholeBody. Raw Dataset includes 1601 TIF images (each 1931 x 1509 x 1 voxel at 0.1216165 mm resolution, derived from 1601 scan projections), xtek and vgi files for volume reconstruction. and Scan of specimen ummz:mammals:163808 (Crocuta crocuta) - WholeBody. Reconstructed Dataset includes 1973 TIF images (each 1931 x 1509 x 1 voxel at 0.121617 mm resolution, derived from 1601 scan projections), xtek and vgi files for volume reconstruction.
Scan of specimen ummz:mammals:170469 (Addax nasomaculatus) - WholeBody. Raw Dataset includes 1601 TIF images (each 1172 x 980 x 1 voxel at 0.08671137 mm resolution, derived from 1601 scan projections), xtek and vgi files for volume reconstruction. and Scan of specimen ummz:mammals:170469 (Addax nasomaculatus) - WholeBody. Reconstructed Dataset includes 1917 TIF images (each 1172 x 980 x 1 voxel at 0.086711 mm resolution, derived from 1601 scan projections), xtek and vgi files for volume reconstruction.
Scan of specimen ummz:mammals:170489 (Chrysocyon brachyurus) - WholeBody. Reconstructed Dataset includes 1495 TIF images (each 1908 x 1243 x 1 voxel at 0.121618 mm resolution, derived from 1601 scan projections), xtek and vgi files for volume reconstruction. and Scan of specimen ummz:mammals:170489 (Chrysocyon brachyurus) - WholeBody. Raw Dataset includes 1601 TIF images (each 1908 x 1243 x 1 voxel at 0.1216177 mm resolution, derived from 1601 scan projections), xtek and vgi files for volume reconstruction.
Scan of specimen ummz:mammals:176889 (Cystophora cristata) - WholeBody. Raw Dataset includes 1601 TIF images (each 1352 x 1999 x 1 voxel at 0.1103929 mm resolution, derived from 1601 scan projections), xtek and vgi files for volume reconstruction. and Scan of specimen ummz:mammals:176889 (Cystophora cristata) - WholeBody. Reconstructed Dataset includes 1113 TIF images (each 1352 x 1999 x 1 voxel at 0.110393 mm resolution, derived from 1601 scan projections), xtek and vgi files for volume reconstruction.
Scan of specimen ummz:mammals:124693 (Aotus azarae) - WholeBody. Reconstructed Dataset includes 1831 TIF images (each 1634 x 1453 x 1 voxel at 0.035184 mm resolution, derived from 1601 scan projections), xtek and vgi files for volume reconstruction. and Scan of specimen ummz:mammals:124693 (Aotus azarae) - WholeBody. Raw Dataset includes 1601 TIF images (each 1634 x 1453 x 1 voxel at 0.03518376 mm resolution, derived from 1601 scan projections), xtek and vgi files for volume reconstruction.
This dataset contains a typical example of Seq-Scope dataset that investigators would generate after examining the initial sequence data. If the quality of the initial Seq-Scope dataset looks great, one may decide to sequence the library much more deeply to maximize the information content. (see NovaScope tutorial at https://seqscope.github.io/NovaScope/ for more details).
The spatial barcode sequences and the initial version of the sequenced cDNA library are deposited separate repository, which is described in the NovaScope documentation ( https://seqscope.github.io/NovaScope/). This data repository contains a deeper sequencing of the same library. A shallower version of sequencing, which does not have overlapping data with the current repository can be found at https://doi.org/10.5281/zenodo.10840695
Yongsung Kim, Weiqiu Cheng, Chun-Seok Cho, Yongha Hwang, Yichen Si, Anna Park, Mitchell Schrank, Jer-En Hsu, Jingyue Xi, Myungjin Kim, Ellen Pedersen, Olivia I. Koues, Thomas Wilson, Goo Jun, Hyun Min Kang2, Jun Hee Lee, “Seq-Scope Protocol: Repurposing Illumina Sequencing Flow Cells for High-Resolution Spatial Transcriptomics” (submitted)