Bacteria live in a broad range of environmental temperatures that require adaptations of their RNA sequences to maintain function. Riboswitches are regulatory RNAs that change conformation upon binding of typical metabolite ligands to control bacterial gene expression. The paradigmatic small class-I preQ1 riboswitches from the mesophile Bacillus subtilis (Bsu) and the thermophile Thermoanaerobacter tengcongensis (Tte) adopt similar pseudoknot structures when bound to preQ1. Here, we use single-molecule detected chemical denaturation by urea to compare the thermodynamic and kinetic folding properties of the two riboswitches, and the urea-countering effects of trimethylamine N-oxide (TMAO). This data includes the experimental findings and associated analyses detailed in the research article titled "Single-molecule FRET observes opposing effects of urea and TMAO on structurally similar meso- and thermophilic riboswitch RNAs". The data consists of multiple zip files, each representing an experiment that corresponds to the key results in the publication. Each experiment includes movies, qualifying smFRET trajectories, and analysis files related to various conditions within that experimental group.
This is a collection of photos of villages located primarily in Central Mali. These photos are primarily of Dogon villages, but there are village photos of other nearby ethnicities, including Bangande, Fulbe, Tuareg, Songay, and Bozo.These photos were taken to document the villages Professor Jeffrey Heath worked in and people he worked with while documenting languages throughout the region. For interactive geographical maps involving these villages see: http://dogonlanguages.org/geography.cfm.
images of plants in nature or specimens, family Anacardiaceae. Includes Anacardium (cashew) and Mangifera (mango). Genus Ozoroa was included in Heeria in the past.
images of plants in nature or specimens, family Annonaceae. Annona reticulata and A. squamosa are planted in courtyards for their fruits (sweetsop). Xylopia is an imported spice ("grains of Selim").
