The datasets associated with this deposit are micro-CT data. These data were generated to be better understand how diffusion iodine contrast enhanced (diceCT) CT scanning can differ amongst major groups of vertebrates. This study stained fish, mammals, reptiles, and amphibians with either H20-Lugol's iodine or Ethylic-Lugol's iodine and measured the differences in the resulting stained soft tissue contrast levels and iodine uptake.