The research described here includes a combination of in vivo animal studies and in vitro cell studies. The animal studies were conducted in NSG mice purchased from Jackson Laboratories and involve implantation of a cell-laden scaffold, animal monitoring, and scaffold explantation. After explantation, scaffolds could be analyzed using PCR or staining. The in vitro cell studies involved administration of Exendin-4 during differentiation of hPSC-derived beta cells and had endpoints such as PCR, flow cytometry, and staining.
Citation to related publication:
Crumbley, Bealer, Lietzke, Soleimanpour, Shea. Exendin-4 enhances insulin-positive phenotype of human pluripotent stem cell-derived beta cells during transplantation. In preparation.
