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  • Quantitative Fluorescence Images of Healthy Human Eyes

    work
    Creator:
    Herzog, Joshua M, Verkade, Angela, and Sick, Volker
    Description:
    Data deposited here includes 60 image sets (30 individual participants, and 2 eyes per individual) consisting of raw fluorescence images, diffuse reflection images using ambient lighting, images used for correction, and calibration, and metadata. Images are split into two wavelength bands as described in the methodology. Raw images are stored in Hierarchical Data Format 5 (HDF5) file nodes (one file per eye) and each image node contains a tag for frame rate, exposure duration, and timestamp (stored in ImageData.zip). Summary statistics including demographic data, participant-reported diseases (e.g., diabetes, keratoconus), and pupil size are also stored in a text-based comma-separated table and as an Excel spreadsheet. Finally, 2-channel pseudocolor images and ratiometric grayscale images combining the two fully-processed image bands are stored as portable network graphics (PNG) files (stored in PseudocolorImages.zip).
    Keyword:
    Fluorescence, Imaging, Ocular lens, Corneal disease, Cataract, and Diabetes
    Citation to related publication:
    Herzog, Joshua M., Verkade, Angela, and Sick, Volker. "Corneal shadowgraphy: a simple, low-cost, rapid, and quantitative tool with potential clinical utility." Manuscript in review. 2024. and Herzog, Joshua M., Verkade, Angela, and Sick, Volker. "Quantitative and rapid in vivo imaging of human lenticular fluorescence." Manuscript in review. 2024.
    Discipline:
    Health Sciences and Engineering