Publicly accessible short-read RNA sequencing (SRS) of clinical exercise studies were extracted from the Gene Expression Omnibus. Oxford Nanopore long-read RNA sequencing (LRS) was performed on mouse gastrocnemius before and following treadmill exercise. Differential gene expression (DGE), differential alternative splicing (DAS), and differential isoform expression (DIE) were analyzed. Gel-based/droplet digital RT-PCR and western blots were performed to validate expression changes of select genes. Both SRS and LRS illustrated significant DGE in skeletal muscle post-exercise, whereby 89 RBPs were significantly up-/down-regulated. rMATS analysis of SRS data revealed that exon-skipping and intron-retaining splicing events were the most common. Swan analysis of LRS data revealed 61 RBPs with significant isoform switching: one of these RBPs, mHnrnpa3, underwent a significant non-coding to protein-coding switch. HnRNP-A3 protein levels validated nearly two-fold increases at 1 hour and 24 hours post-exercise.
Impact of Acute Endurance Exercise on Alternative Splicing in Skeletal Muscle. Alexander Ahn, Jeongjin J. Kim, Aaron L. Slusher, Jeffrey Y. Ying, Eric Y. Zhang, Andrew T. Ludlow bioRxiv 2024.11.21.624690; doi: https://doi.org/10.1101/2024.11.21.624690
