Search Results

Search Constraints

Start Over
Filtering by: Keyword inflammation Remove constraint Keyword: inflammation

Search Results

  • Effect of thermoneutral housing on a murine sepsis model

    work
    Creator:
    Jean Nemzek and Chris Fry
    Description:
    After an initial acclimation period at standard temperatures, mice were randomly placed in static cages (3-5 mice/cage) in specialized climate chambers (Powerscientific, Pipersville, PA). Chamber temperatures were set to either 22°C or 30°C with relative humidity set to 30%. After 7 days, groups of mice (n=10/group) were euthanized without further manipulations to establish the effects of ambient temperature on select markers of inflammation including cell counts and cytokine concentrations in plasma and peritoneal lavage fluid. Spleen cells were harvested for total counts and in vitro stimulation. Additional groups of mice exposed to either 22°C or 30°C underwent cecal ligation and puncture surgery to induce polymicrobial peritonitis, then returned to their assigned housing temperature. Survival was monitored for 7 days after surgery. In a separate cohort of mice, inflammatory responses at 6 hours after surgery were examined in the systemic (blood) and local (peritoneal lavage) compartments. the experiment was repeated with mice implanted with a thermistor to monitor body temperature over 72 hours.
    Keyword:
    sepsis, ambient temperature, and inflammation
    Citation to related publication:
    Chan G, et al. Impact of thermoneutral acclimation on a murine model of polymicrobial peritonitis. Under review.
    Discipline:
    Health Sciences

  • Supplemental Data for "Enduring and sex-specific changes in hippocampal gene expression after a subchronic immune challenge"

    work
    Creator:
    Tronson, Natalie C and Tchessalova, Daria
    Description:
    The main goal of this research was to identify potential molecular pathways that contribute to memory dysregulation and decline that persists long after illness or inflammation. We have previously established a subchronic immune challenge model that results in memory impairments months after the inflammatory challenge. This project aimed to determine whether memory impairments were accompanied by transcriptional dysregulation in memory related brain region (the hippocampus). These data show the differential gene expression as log2fold change (and p-value) in males and females 3 months after immune challenge (Supp Tables 1 and 2); after a subsequent immune challenge (Supp Tables 3 and 4); the differential regulation of genes in males and females (Supp Table 5); genes differentially expressed in the hippocampus of males and females at baseline (Supp Table 6) and the differential regulation of those genes in males and females after immune challenge (Supp Tables 7,8).
    Keyword:
    hippocampus, lipopolysaccharide, differential gene expression, RNA sequencing, neuroimmune, sex differences, learning and memory, and inflammation
    Citation to related publication:
    Tchessalova, D., & Tronson, N. C. (2019). Enduring and sex-specific changes in hippocampal gene expression after a subchronic immune challenge. BioRxiv, 566570.  https://doi.org/10.1101/566570
    Discipline:
    Science and Health Sciences