DNA was extracted from previous stored vaginal slides; and DNA was pre-amplified using universal bacterial primers based on 16s rDNA; total bacterial and species specific bacterial copy numbers were identified using total bacteria primers and primers specific to each bacteria taxa; samples with copy numbers fewer than 100 were under the limit of detection and were considered to be negative
This is the bacterial DNA data extracted from the gram stain slides. The targeted bacteria genera and species include: Atopobium spp., bacterial vaginosis-associated bacterium (BVAB) types 1, 2 and 3 in the order Clostridiales, Escherichia coli, Gardnerella vaginalis, Group B Streptococcus, Lactobacillus spp., Mobiluncus spp., Mycoplasma spp., and Ureaplasma spp. We also used a primer set for Lactic Acid Bacteria (LAB) that includes lactic acid producing bacteria of the genera Lactobacillus, Pediococcus, Leuconostoc, and Weissella. We calculated the relative proportion of each bacterial taxon using the bacterial copies measured by each specific bacteria primer divided by the total bacterial copies. The limit of detection was 100 copies and readings lower than the limit were considered negative
To Cite this Work:
PTB microbial data [Data set]. University of Michigan - Deep Blue.
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