Work Description

Title: Concentrations and isotope values of nitrogen forms during 2020 Lake Erie Cyanobacterial harmful algal bloom Open Access Deposited

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Attribute Value
Methodology
  • Water samples were collected weekly from Lake Erie during the cyanobacterial harmful algal bloom. Particulate organic matter was collected by vacuum filtering water through pre-combusted filters. Filters were frozen at -80°C, and the filtrate was subsequently filtered through 0.2 µm polyethersulfone flat filters and collected for nutrient and fluorescence analysis of dissolved organic matter. Filtered samples were analyzed for concentrations of nitrate, ammonium, and dissolved organic nitrogen. 15N natural abundance isotope analysis of filtered samples was carried out using the denitrifier method, yielding measurements of nitrate and dissolved organic nitrogen.
Description
  • Nitrogen availability is hypothesized to play a central role in cyanoHAB progression, as well as production of the N-rich toxin microcystin. Recent work implicated reduced N substrates like ammonium and dissolved organic N (DON) in promoting greater bloom biomass and longevity. To examine the relative importance of oxidized and reduced N substrates to phytoplankton during different bloom stages, we measured concentrations and natural abundance δ15N isotope values of dissolved N substrates and phytoplankton biomass throughout the entirety of the 2020 cyanoHAB in Western Lake Erie. This is the first data on DON concentrations and isotope values in Western Lake Erie. We measured nitrate concentrations and isotope values to compare with DON and also with particulate organic matter (POM) 15N isotope values. This dataset is described and analyzed in the publication, "Patterns in sources and forms of nitrogen during a freshwater cyanobacterial harmful algal bloom".
Creator
Depositor
Contact information
Discipline
Funding agency
  • National Science Foundation (NSF)
  • Other Funding Agency
Other Funding agency
  • University of Michigan President's Postdoctoral Fellowship Program
Keyword
Date coverage
  • 2020-06-16 to 2020-10-05
Citations to related material
  • Kharbush, Jenan J., Robinson, Rebecca S., Carter, Susan J. In revision. Patterns in sources and forms of nitrogen during a freshwater cyanobacterial harmful algal bloom. Limnology and Oceanography.
Resource type
Last modified
  • 12/06/2022
Published
  • 11/03/2022
Language
DOI
  • https://doi.org/10.7302/mg4b-x721
License
To Cite this Work:
Kharbush, J. J., Carter, S. J., Robinson, R. S. (2022). Concentrations and isotope values of nitrogen forms during 2020 Lake Erie Cyanobacterial harmful algal bloom [Data set], University of Michigan - Deep Blue Data. https://doi.org/10.7302/mg4b-x721

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Files (Count: 3; Size: 40.3 KB)

Thumbnailthumbnail-column Title Original Upload Last Modified File Size Access Actions
2020LakeErie_forDeepBlue_data.csv 2022-11-02 2022-11-02 6.43 KB Open Access
Definitions_of_terms_and_variables.csv 2022-11-03 2022-11-03 3.76 KB Open Access
Readme_2020LakeErie.docx 2022-11-03 2022-11-03 30.1 KB Open Access

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