Work Description

Title: Research data supporting "Flexible Synthesis Scheme and Application of AuNP Surface-Conjugatable Meta-Iodobenzylguanidine Derivatives for Enhanced Cellular Internalization" Open Access Deposited

h
Attribute Value
Methodology
  • Synthetic chemistry and analytical/physical chemistry experiments. Data is reported from outputs including: A.) Synthetic products: NMR spectroscopy, mass spectrometry, B.) Cellular uptake studies: dark-field microscopy and quantitative inductively quantitative mass-spectrometry (qICP-MS), C.) Nanoparticle characterization: transmission electron microscopy (TEM), UV-Vis, dynamic light scattering (DLS), zeta potential. See:  https://doi.org/10.1021/acsmaterialslett.3c00781 for description of synthetic protocols.
Description
  • Research data supporting, 'Flexible Synthesis Scheme and Application of AuNP Surface-Conjugatable Meta-Iodobenzylguanidine Derivatives for Enhanced Cellular Internalization', 10.1021/acsmaterialslett.3c00781, AuNP = Au nanoparticle. In 10.1021/acsmaterialslett.3c00781, we report the synthesis and application of two metaiodobenzylguanidine (MIBG) derivatives decorated on the surface of AuNPs at high molar ratios; greatly enhanced cellular uptake is observed across neuroblastoma (NB), HeLa, and HEK cell lines. This dataset consists of synthetic NMR and mass spec data for the small molecules and their intermediates, dark-field microscopy data, inductively-coupled plasma mass spectrometry data, and raw data for characterizing the AuNPs (TEM, DLS, zeta potential).
Creator
Creator ORCID
Depositor
  • ammclean@umich.edu
Contact information
Discipline
Funding agency
  • National Institutes of Health (NIH)
ORSP grant number
  • R01CA186769
Keyword
Date coverage
  • 2019 to 2022
Citations to related material
  • Flexible Synthesis Scheme and Application of AuNP Surface-Conjugatable Metaiodobenzylguanidine Derivatives for Enhanced Cellular Internalization Natalie S. Potter, Alan McLean, Evan C. Bornowski, Thomas Hopkins, Jingyi Luo, John P. Wolfe, Wei Qian, and Raoul Kopelman ACS Materials Letters Article ASAP DOI: 10.1021/acsmaterialslett.3c00781
Resource type
Last modified
  • 10/18/2023
Published
  • 10/18/2023
Language
DOI
  • https://doi.org/10.7302/mzwr-sg74
License
To Cite this Work:
Potter, N. S., McLean, A., Bornowski, E. C., Hopkins, T., Luo, J., Wolfe, J. P., Qian, W., Kopelman, R. (2023). Research data supporting "Flexible Synthesis Scheme and Application of AuNP Surface-Conjugatable Meta-Iodobenzylguanidine Derivatives for Enhanced Cellular Internalization" [Data set], University of Michigan - Deep Blue Data. https://doi.org/10.7302/mzwr-sg74

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Files (Count: 2; Size: 23.3 MB)

Thumbnailthumbnail-column Title Original Upload Last Modified File Size Access Actions
README.txt 2023-10-15 2023-10-15 4.04 KB Open Access
DATA_10.1021acsmaterialslett.3c00781.zip 2023-10-18 2023-10-18 23.3 MB Open Access

Instructions: Unzip Data_10.1021acsmaterialslett.3c00781.zip.

Date: 15 October 2023

Dataset Title: Research data supporting "Flexible Synthesis Scheme and Application of AuNP Surface-Conjugatable Meta-Iodobenzylguanidine Derivatives for Enhanced Cellular Internalization"

Dataset Creators: N. Potter,† A. McLean,†* E. Bornowski, T. Hopkins, J. Luo, J. Wolfe, W. Qian,* R. Kopelman

Dataset Contact: ammclean@umich.edu, wqian@imra.com

Funding: NIH R01CA186769

Key Points:
- In 10.1021/acsmaterialslett.3c00781 (manuscript DOI for this dataset), we report the synthesis and application of two metaiodobenzylguanidine (MIBG) derivatives decorated on the surface of AuNPs at high molar ratios; greatly enhanced cellular uptake is observed across neuroblastoma (NB), HeLa, and HEK cell lines
- This dataset consists of synthetic NMR and mass spec data for the small molecules and their intermediates, dark-field microscopy data, inductively-coupled plasma mass spectrometry data, and raw data for characterizing the AuNPs (TEM, DLS, zeta potential)

Methodology:
The data are organized figure-by-figure as reported in 10.1021/acsmaterialslett.3c00781. Detailed experimental protocols can be found in Supporting Information S1 of the manuscript (freely and publicly available), including synthesis of the MIBG derivatives, synthesis of the pulsed-laser ablation AuNPs, preparation of the decorated AuNPs, zeta potential measurements, cell culture, dark field microscopy, and quantitative inductively coupled plasma mass spectormetry (qICP-MS)

Instrument and/or Software specifications:

.ppt - for creating art/compiling figures (.odp alternative is also provided for all .ppts)
.xls - Excel files, raw/processed data (.CSVs exported by sheet are also provided with comma delimiters)
.doc - Word files
.opju - raw/processed data in charts/graphs, need Origin software to open, freely available (no license required) Origin Viewer can be used, in all cases accompanies parent file
.mnova - NMR raw data files, need Mestrenova software to read
.cdx - ChemDraw files, need ChemDraw softare to open
.jpg, .png, .tiff, etc. - image files

Files Contained (organized by folder):

00_TOC: Table of contents image for manuscript (PPT/ODP)
01_Figure1: Figure 1 of manuscript, overview figure showing AuNP scheme (PPT/ODP)
02_Figure2: Figure 2 of manuscript, dark-field images (JPG/PNG)
03_Figure3: Figure 3 of manuscript, quantitative ICP-MS data in neuroblastoma (NB) cells of dMIBG/dBG derivatives (and PEGylated control), Excel (raw data), Origin (processed plots)
04_Figure4: Figure 4 of manuscript, quantitative ICP-MS data for 900:1 samples across NB, HeLa, and HEK cell lines (including DMI inhibitor experiments), Excel (raw data), Origin (processed plots)
05_Scheme1: ChemDraw file of synthetic scheme, (CDX)
06_SIData_Figs1-6: Compiled NMR data for dMIBG derivative (mNOVA), mass spec data of each compound (exported to MS Word)
07_SIData_Figs7-9: Compiled NMR data for dBG derivative (mNOVA), mass spec data of each compound (exported to MS Word)
08_SIFig10: TEM image of AuNPs (JPG)
09_SIFig11: Exported UV-Vis data of AuNP, PEGylated control, and dMIBG/dBG-conjugated sample (6000:1 dMIBG shown) (Excel/OPJU)
10_SIFig12: Exported DLS data of AuNP, PEGylated control, and dMIBG/dBG-conjugated sample (6000:1 dMIBG shown) (Excel/OPJU)
11_SIFig13: Exported Zeta potential data of dMIBG-conjugated AuNPs (0:1, 600:1, 1200:1, 2400:1, 4000:1) (Excel/OPJU, graphs exported to PPT/ODP)
12_SIFig14: Exported Zeta potential data of dBG-conjugated AuNPs (0:1, 600:1, 1200:1, 2400:1, 4500:1) (Excel/OPJU, graphs exported to PPT/ODP)

Use and Access:
This data set is made available under Creative Commons 4.0.

To Cite Data:
Potter, N. S., McLean, A., Bornowski, E. C., Hopkins, T., Luo, J., Wolfe, J. P., Qian, W., Kopelman, R. Research data supporting "Flexible Synthesis Scheme and Application of AuNP Surface-Conjugatable Meta-Iodobenzylguanidine Derivatives for Enhanced Cellular Internalization" [Data set], University of Michigan - Deep Blue Data. https://doi.org/10.7302/mzwr-sg74

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