Now showing items 31-40 of 285
Site‐Specific Fluorescent Labeling of Large RNAs with Pyrene
(Oxford University PressWiley Periodicals, Inc., 2004-12)
Pyrene is a useful chromophore for monitoring the tertiary structure and folding of large RNAs. This unit describes the general preparation of a large RNA (>80 nucleotides in length) that has been site‐specifically modified ...
Preparation of 2′‐O‐[(Triisopropylsilyl)oxy]methyl‐protected Ribonucleosides
(Oxford University PressWiley Periodicals, Inc., 2001-12)
The [(triisopropylsilyl)oxy]methyl (TOM) group is a useful protecting group for the 2′‐OH of ribonucleosides to be used for oligoribonucleotide synthesis by the phosphoramidite method. It is completely stable to all reaction ...
A study of a C Α, Β -didehydroalanine homo-oligopeptide series in the solid-state by 13 C cross-polarization magic angle spinning NMR
(John Wiley & Sons, Ltd., 2004-06)
The fully extended peptide conformation (2.0 5 -helix) has been investigated for the first time in the solid-state by 13 C cross-polarization magic angle spinning NMR. The compounds examined are members of a terminally ...
Synthesis of Phosphorothioate Oligonucleotides with Stereodefined Phosphorothioate Linkages
(Wiley‐Liss, 2003-09)
A method for solid‐phase synthesis of stereodefined PS‐oligos via an oxathiaphospholane approach using pure P‐diastereomers of nucleoside oxathiaphospholane monomers is described. The oxathiaphospholane monomers are ...
Chemotherapy for prostate cancer: implementing early systemic therapy to improve outcomes
(Springer-Verlag, 2005-11)
Prostate cancer remains a significant health concern for men in the USA as it is a leading cancer diagnosis and a cause of death. With the use of prostate-specific antigen or screening, a stage migration has occurred with ...
Protection of 5′‐Hydroxy Functions of Nucleosides
(IRL PressWiley Periodicals, Inc., 2000-02)
The 5‐hydroxy group is the primary hydroxy group of nucleosides. It is mandatory to protect 5‐hydroxyls in all methods of oligonucleotide synthesis that require nucleoside synthons. This unit discusses a wide variety of ...
Proteomic analysis of cold adaptation in a Siberian permafrost bacterium – Exiguobacterium sibiricum 255–15 by two-dimensional liquid separation coupled with mass spectrometry
(WILEY-VCH Verlag, 2006-10)
Bacterial cold adaptation in Exiguobacterium sibiricum 255–15 was studied on a proteomic scale using a 2-D liquid phase separation coupled with MS technology. Whole-cell lysates of E. sibiricum 255–15 grown at 4°C and ...
Characterization of Tertiary Folding of RNA by Circular Dichroism and Urea
(Wiley Periodicals, Inc., 2001-04)
CD spectroscopy can be used to monitor RNA tertiary folding transitions that may not be observable by absorbance spectroscopy. With the use of computer‐controlled titrators, data can be acquired rapidly, and accurate ...
Biophysical Analysis of Nucleic Acids
(VCH PublishersWiley Periodicals, Inc., 2000-02)
This overview unit provides a thorough overview of biophysical methods used for structure analysis, including X‐ray diffraction, nuclear magnetic resonance, optical spectroscopy, theoretical and computational methods, and ...