Now showing items 11-20 of 285
Probing RNA Structure with Chemical Reagents and Enzymes
(Wiley Periodicals, Inc., 2000-02)
This unit provides thorough coverage of the most useful chemical and enzyme probes that can be used to examine RNA secondary and tertiary structure. Footprinting methods are presented using dimethyl sulfate, diethyl ...
Oligoribonucleotides with 2′‐O‐(tert‐Butyldimethylsilyl) Groups
(Wiley Periodicals, Inc.IRL Press,, 2000-07)
The chemical synthesis of oligoribonucleotides on solid support is routinely performed via the phosphoramidite method. However, the additional 2‐OH function of the ribofuranosyl sugar requires suitable protection during ...
Determination of Binding Mode: Intercalation
(Academic PressWiley Periodicals, Inc., 2000-02)
A small molecule can be assumed to bind to DNA by intercalation between base pairs if it causes lengthening and unwinding of the DNA helix and undergoes changes in its spectral properties, such as DNA‐induced hypochromism ...
Early events of Bacillus anthracis germination identified by time-course quantitative proteomics
(WILEY-VCH Verlag, 2006-10)
Germination of Bacillus anthracis spores involves rehydration of the spore interior and rapid degradation of several of the protective layers, including the spore coat. Here, we examine the temporal changes that occur ...
Cellular Delivery of Locked Nucleic Acids (LNAs)
(Wiley Periodicals, Inc., 2002-06)
Locked nucleic acids (LNAs) are RNA derivatives that have an O‐methylene linkage between the 2 and 4 positions of the ribose. This leads to exceptionally high‐affinity binding to complementary sequences. They are synthesized ...
Prevention of visual stress and migraine with precision spectral filters
(Wiley Subscription Services, Inc., A Wiley Company, 2007-11)
Individuals who suffer migraine, particularly migraine with visual aura (MwA), are susceptible to physiologically strong visual stimuli and find them aversive. Strong stimuli including bright light and certain visual ...
Low-Temperature SCR of NO with NH 3 over USY-Supported Manganese Oxide-Based Catalysts
(Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media, 2003-04)
A series of catalysts of manganese oxide, manganese–cerium and iron–manganese oxide supported on USY (ultra-stable Y zeolite) were studied for the low-temperature selective catalytic reduction (SCR) of NO with ammonia in ...
Angiotensin Converting Enzyme Inhibitors: Should They be Used Early Post Myocardial Infarction?
(Kluwer Academic Publishers; Springer Science + Business Media, Inc., 2005-03)
Base Composition Analysis of Nucleosides Using HPLC
(Applied Biosystems,Wiley Periodicals, Inc., 2000-07)
In this protocol, nuclease digestion of an oligonucleotide is followed by dephosphorylation and HPLC analysis of the monomers on a reversed‐phase C18 column. This method can be used to detect and quantitate a wide variety ...